慢性间歇低氧对大鼠认知功能及大脑环磷酸腺苷反应单元结合蛋白表达的影响  

Effects of Chronic Intermittent Hypoxia on Rat’s Cognition and Expression of cAMP Response Element Binding Protein in Brain

作　　者：康晶[1] 王月华[2] 何静雅[1] 李清泉[1] 胡克[1] 

机构地区：[1]武汉大学人民医院呼吸科,湖北武汉430060 [2]湖北省襄樊市中心医院,湖北襄樊441021

出　　处：《武汉大学学报（医学版）》2011年第1期5-7,55,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81070065)

摘　　要：目的:探讨慢性间歇低氧对大鼠认知功能及大脑环磷酸腺苷反应单元结合蛋白(CREB)表达的影响。方法:通过常压低氧仓建立间歇低氧大鼠模型,即对成年大鼠每90s交替给予10%氧和21%氧,每天8h,连续处理14d后,以Morris水迷宫观察大鼠空间学习记忆的变化,并采用RT-PCR法观察大鼠皮层区及海马区环磷酸腺苷反应单元结合蛋白mRNA的表达。结果:与常压对照组比较,常压间歇低氧组大鼠在Morris水迷宫中的空间学习记忆能力明显下降,表现为平均逃避潜伏期延长和游泳总距离增加。同时,大鼠皮层区及海马区CREBmR-NA的表达水平明显降低。结论:慢性间歇低氧可引起大鼠空间学习记忆能力下降,而大鼠这种慢性脑功能损害可能与大脑CREBmRNA的表达下调有关。Objective： To study the effect of chronic intermittent hypoxia on cognition and expression of cAMP response element binding protein （CREB） in rats. Methods： Chronic intermittent hypoxia rat model was established by a normal pressure and low oxygen cabin. Rats were alternately dealt with 10% and 21% oxygen every 90 seconds for 14 days, and 8 hours each day. Then the spatial reference task learning was assessed by the Morris water maze and the expression of CREB mRNA was determined with RT-PCR.Results： Compared with the normal oxygen control group, rats exposed to normal pressure and chronic intermittent hypoxia showed significant impairment in their cognitive function, such as learning-memory performance in Morris water maze, in which, both the average escape latencies and swim path distances increased. The expressions of CREB mRNA in cortex and hippocampus were lower than those in control group. Conclusion： Chronic intermittent hypoxia can impair rat spatial reference task learning and the chronic brain function damage may be related to the lower expression of CREB mRNA.

关 键 词：慢性间歇低氧 认知功能 环磷酸腺苷反应单元结合蛋白 

分 类 号：R363.21[医药卫生—病理学]