Endostar联合放疗对Lewis肺癌小鼠生长及AQP1、HIF-1α mRNA表达的影响  

Effect of Endostar Combined with Radiotherapy on the Expression of Aquaporin 1 and Hypoxia-Inducible Factor-1 Alpha mRNA and Growth of Mice with Lewis Lung Cancer

作　　者：张金忠[1] 胡科[1] 戈伟[1] 李长虎[1] 郑永法[1] 张令[1] 王慧敏[1] 曹德东[1] 

机构地区：[1]武汉大学人民医院肿瘤诊疗中心,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2011年第1期44-48,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30970860)

摘　　要：目的:探讨Endostar联合放射治疗对Lewis肺癌小鼠移植瘤生长及介导氧跨膜转运的水通道蛋白1(AQP1)及乏氧诱导因子-1α(HIF-1α)mRNA表达的影响。方法:将32只Lewis肺癌移植瘤小鼠随机分为4组:空白对照组、单用Endostar组(ES组)、单用放疗组(RT组)、Endostar联合放疗组(ES+RT组),对各组肿瘤组织中AQP1及HIF-1αmRNA的表达情况进行检测。结果:AQP1mRNA的表达:与对照组相比,ES组变化无统计学差异(P>0.05)、RT组降低(P<0.05)、ES+RT组升高(P<0.05);HIF-1αmRNA的表达:与对照组相比,ES组变化无统计学差异(P>0.05)、RT组与ES+RT组均降低(P<0.05)。Pearson分析AQP1mRNA与HIF-1αmRNA的表达呈负相关(r=-0.58,P<0.01)。结论:Endostar联合放疗可能通过上调AQP1mRNA表达,增加细胞摄氧量,抑制HIF-1αmRNA表达,而增强射线对肿瘤细胞的杀伤作用。Objective： To explore the effect of Endostar combined with radiotherapy on the mRNA expression of aquaporin 1 （AQP1） which mediated transmembrane transport of oxygen and hypoxia-inducible factor-1α （HIF-1α） in tumor-bearing mice with Lewis lung cancer. Methods： Thirty-two tumor-bearing mice were randomly divided into four groups： control group, Endostar group, radiation therapy group, and Endostar combined with radiotherapy group. After treatment, the tumor growth curve was calculated, and the real time PCR was used to detect the different expression of AQP1 and HIF-1α mRNA in each group. Results： Compared with that in control group, the expression of AQP1 mRNA had no changes in Endostar group （P0.05）, decreased in radiotherapy group （P0.05）, and increased in Endostar combined with radiotherapy （P0.05）. Compared with control group, the expression of HIF-1α mRNA had no changes in Endostar group （P0.05）, and was lower both in radiotherapy group and Endostar combined with radiotherapy group （both P0.05）. There existed negative correlation between the expression of AQP1 and HIF-1α mRNA （r=-0.58,P0.01）. Conclusion： Endostar combined with radiotherapy may enhance the tumor killing effect of radiation therapy through increasing AQP1 mRNA expression and inhibiting the HIF-1α mRNA expression, which enhances the oxygen uptake of cells, thereby increases the killing effect of radiation on tumor cells.

关 键 词：ENDOSTAR 放射治疗 水通道蛋白1 乏氧诱导因子-1Α 

分 类 号：R73-352[医药卫生—肿瘤]