5-羟色胺2A受体阻断剂对大鼠肝脏再生的影响  

Effect of serotonin receptor 2 blockage on liver regeneration after partial hepatectomy

作　　者：杨仙珊[1] 张晶[1] 李晓晴[1] 丁美[1] 赵军[1] 郑素军[1] 段钟平[1] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝中心,北京100069

出　　处：《胃肠病学和肝病学杂志》2011年第1期71-73,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家"十一五"科技重大专项课题<乙肝重型肝炎肝衰竭优化诊疗方案>(2008ZX10002-005-3);国家"十一五"科技重大专项课题<北京市朝阳区艾滋病和病毒性肝炎等重大传染病综合防治示范区建设研究>(2009ZX10004-903);北京市科委课题<北京朝阳区重大传染病防控示范区乙肝;艾滋病治疗与干预研究>(D08050703090903)

摘　　要：目的观察5-羟色胺2A受体阻断剂酮色林对大鼠肝部分切除后肝再生的影响,了解5-羟色胺及其受体在肝脏再生中的作用。方法 80只雄性Wistar大鼠随机分为实验组和对照组。采用肝大部分切除术建立肝再生模型,术后16 h分别给予腹腔内注射酮色林(实验组)和生理盐水(对照组),采用免疫组化及流式细胞技术动态观察并比较两组大鼠术后24、36、48、72 h肝脏Ki67、增殖细胞核抗原的表达情况。结果大鼠肝大部切除术后24、36 h肝脏表达Ki67、增殖细胞核抗原最为活跃,而后表达逐渐下降。实验组大鼠肝脏表达Ki67、增殖细胞核抗原较对照组显著下降(P<0.05)。结论 5-羟色胺2A受体阻断剂酮色林显著抑制大鼠肝大部切除术后的肝脏再生,说明5-羟色胺具有一定的促进肝再生的作用,2A受体是其重要的信号传导受体之一。Objective To study the effect of serotonin receptor 2 blockage（ketanserin） on liver regeneration after 60%～70% partial hepatectomy（PH）. Methods Eighty male Wistar rats were randomly divided into two groups,all rats were subjected to 60%～70% PH.Rats in the control group were given 0.9% saline by intraperitoneal injection,while the rats in experimental group were given ketanserin at 16 hours after PH.All rats were sacrificed at 24 h,36 h,48 h and 72 h after PH.Liver regeneration was evaluated by the immunochemical staining of Ki67 and the flow cytometry detection of proliferating cell nuclear antigens（PCNA）. Results Liver regeneration peaked at 24 h and 36 h after 60%～70% PH in rats.The expression of Ki67 and PCNA decreased significantly in experimental group than those in control group. Conclusion Serotonin receptor 2 blockage can arrest significantly the liver regeneration.The results also demonstrated that serotonin may be one of the key biomolecules for promoting the liver regeneration by binding to its receptor 2.

关 键 词：肝大部切除术 酮色林 5-羟色胺 肝再生 

分 类 号：R575[医药卫生—消化系统]