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作 者:胡赟[1] 郑雷蕾 唐甜[3] 赵志河[3] 宋锦璘 邓锋
机构地区:[1]重庆医科大学附属口腔医院口腔预防与儿童牙病科 [2]正畸科,重庆400015 [3]四川大学华西口腔医院正畸科,成都610041
出 处:《华西口腔医学杂志》2011年第1期17-20,26,共5页West China Journal of Stomatology
基 金:国家自然科学基金资助项目(81000463和10902075);高等学校博士学科点专项科研基金新教师类基金资助项目(200955031-20006)
摘 要:目的研究微种植体周围炎对微种植体-骨界面的组织学改变,同时对龈沟液相关细胞因子进行检测,从而探讨微种植体周围炎的发生机制。方法健康雄性Beagle纯种犬4只,采用自身对照,随机选定实验动物一侧为对照组,一侧为实验组,每侧植入4枚微种植体。实验组种植体颈部结扎丝线,诱导微种植体周围炎。在植入后第1、2、3、4周分别处死动物,处死前收集种植体周围龈沟液(PISF),酶联免疫吸附试验(ELISA)检测肿瘤坏死因子-α(TNF-α)含量,收集标本做硬组织切片观察。结果根据植入后时间的延长,实验组微种植体颈部表现明显的进展性破坏:植入1周微种植体颈部聚集大量炎性细胞,但并没有破坏皮质骨;植入2周微种植体颈部皮质骨已经出现少量角形吸收;植入3周表现骨吸收进一步向纵深发展;植入4周表现骨吸收已经至第二螺纹,充满大量胶原纤维。植入后1、2、3、4周,实验组与对照组PISF和TNF-α相比均有显著性差异(P<0.05),实验组高于对照组;实验组植入后4周PISF和TNF-α显著高于其余各阶段(P<0.05)。结论微种植体周围炎首先破坏结合界面颈部,并沿界面进一步纵深发展。无论是健康或者炎症的微种植体周围都有TNF-α表现,炎症的发生可能是造成TNF-α含量上调的始动因素,炎症的发生进一步加剧了TNF-α表达的上调。Objective To study the histological change of microscrew-bone interface,detect the relative cytokine of gingival crevicular fluid,and explore the impossible mechanism of peri-implantitis.Methods Four male Beagles were collected.Randomly select one side of animals jaw as the experimental group to induce the peri-implantitis,and another side as the controll group.Four microscrews were implanted on each side.In the 1st,2nd,3rd,4th weeks after implantation,collect peri-implant sulcular fluid(PISF) and detect tumor necrosis factor-α(TNF-α) levels before sacrificed,and the harvest tissue were observed in histological ways.Results According to the extension of time after implantation,the experimental group showed visible progress of interface destruction:1st week after implantation showed large numbers inflammatory cells collected at the neck but did not undermine the cortical bone;2nd week after implantation,cortical bone were observed angular absorption;Bone resorption continued to develop and at the 4th week,bone resorption were enlarged to the second thread of microscrew and the interface was filled with a large number of collagen fibers.Conclusion The destruction of interface began at the neck of microscrew,and the further development was along the interface in depth.Both microscrews with peri-implantitis and the healthy controls showed the presence of TNF-α.Inflammation accumulation might trigger the up-regulation of TNF-α level,and the onset of inflammation would enhance the up-trend.
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