鼠双微粒体2蛋白、P53在口腔白斑和鳞癌中的表达及其相关性的研究  

Expression and its relationship of murine double minute 2 and P53 protein in oral leukoplakia and squamous cell carcinoma

作　　者：韩帮锋[1] 吴平凡[1] 潘剑[1] 李一[1] 李龙江[1] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,成都610041

出　　处：《华西口腔医学杂志》2011年第1期79-82,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30973345和30471901)

摘　　要：目的研究鼠双微粒体2(MDM2)和P53在口腔白斑及鳞癌中的表达及其相关性。方法采用免疫组织化学SP方法对15例正常口腔黏膜组织、24例口腔白斑(OLK)组织及41例口腔鳞癌组织中的MDM2蛋白和P53蛋白进行检测。结果正常黏膜中未见MDM2和P53阳性表达,口腔白斑和口腔鳞癌组织中MDM2阳性率分别为58.3%和75.6%,P53的阳性率分别为37.5%和68.3%,与正常组相比均具有显著性差异(P<0.05)。口腔白斑和口腔鳞癌组相比,MDM2阳性率没有显著性差异(P>0.05),P53阳性率具有显著性差异(P<0.05)。两种指标进一步的相关性分析显示,MDM2与P53蛋白在口腔白斑组(P=0.018)及口腔鳞癌组(P=0.000)中均呈现正相关的关系。结论 MDM2在口腔白斑和口腔鳞癌中的高表达提示该基因在口腔鳞癌的发生发展中起显著作用,其与P53表达的相关性表明这二者可能在口腔鳞癌的形成过程中起协同作用。Objective To study the expression and its relationship of murine double minute 2（MDM2）,P53 protein in oral leukoplakia and squamous cell carcinoma.Methods Immunohistochemical assay was used to detect the expression of MDM2 and P53 proteins in 15 normal oral epithelium tissues,24 cases of oral leukoplakias and 41 cases of oral squamous cell carcinomas.Results There were no positive expression of MDM2 and P53 in normal mucosa.With significantly difference compared with the normal group（P0.05）,the positive rates of MDM2 and P53 were 58.3%,75.6% and 37.5%,68.3% in oral leukoplakia and squamous cell carcinoma.MDM2-positive rate had no significant difference（P0.05）,while P53-positive rate was significant difference（P0.05） compared with oral leukoplakia and oral squamous cell carcinoma.Two kinds of indicators for further correlation analysis showed that MDM2 and P53 protein showed a positive correlation relationship both in oral leukoplakia（P=0.018） and oral squamous cell carcinoma（P=0.000）.Conclusion The expression of MDM2 in oral leukoplakia and oral squamous cell carcinoma suggests that this gene may play a significant role in the process of carcinogenesis of oral squamous cell carcinoma.The correlation of MDM2 and P53 expression indicates that both of them may play a synergistic role in the process of carcinogenesis of oral squamous cell carcinoma.

关 键 词：鼠双微粒体2 P53蛋白 口腔白斑 口腔鳞状细胞癌 

分 类 号：R739.8[医药卫生—肿瘤]