高浓度氧增加未成年大鼠肺血红素加氧酶-1表达  

Hyperoxia increases the expression of lung heme oxygenase-1 in rat pups

作　　者：吴长毅[1] 岳峰[1] 李民[1] 张利萍[1] 王军[1] 郭向阳[1] 

机构地区：[1]北京大学第三医院麻醉科,北京100191

出　　处：《基础医学与临床》2011年第2期124-127,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30801075)

摘　　要：目的探讨高浓度氧对未成年大鼠肺血红素加氧酶-1(HO-1)表达的影响。方法将生后21 d SD大鼠40只随机分为空气组和12、24、48及72 h高氧组,分别置于空气和常压高氧箱(92%～94%O2)中。检测左肺湿/干重比及肺组织病理学改变,用RT-PCR和Western blot法分别检测肺HO-1 mRNA及HO-1蛋白表达情况。结果高氧12 h组肺HO-1 mRNA吸光度积分相对值和高氧24 h组HO-1蛋白表达水平分别为0.350±0.043和0.455±0.046,较对照组0.263±0.037和0.280±0.044明显升高,且均随高氧暴露时间延长而表达进一步增加(P<0.05,P<0.01)。与空气组比较,高氧48 h组和高氧72 h组左肺湿重/干重及肺损伤评分显著增高(P<0.05,P<0.01)。结论高浓度氧可引起未成年大鼠肺组织HO-1表达增多。Objective To investigate the effects of hyperoxia on the expression of lung heme oxygenase-1 （ HO-1 ） in rat pups. Methods 21-d-old male Sprague-Dawley rat pups were randomly divided into 5 groups： room-air control group and 12, 24, 48, 72 hours hyperoxia groups. The rat pups were continuously exposed to oxygen （92% - 94% ） and normal atmosphere respectively. The left lung wet/dry weight ratio and the histological examination were performed. The expressions of lung HO-1 mRNA and HO-1 protein were also measured by RT-PCR and Western blot, respectively. Results The expressions of lung HO-1 mRNA in 12 hours hyperoxia group （0. 350 ± O. 043 ） and HO-1 protein in 24 hours hyperoxia group（0. 455±0. 046） were increased as compared to those in the normal atmosphere （0. 263 ± 0. 037, 0. 280± 0. 044） , and the increase was more significant with the longer hyperoxia exposure （P 〈0. 05, P 〈0. 01 ）. Compared with the normal atmosphere control, the left lung wet/dry weight ratio and the lung injury scores was significantly increased in 48 hours hyperoxia group and 72 hours hyperoxia group （P 〈0. 05, P 〈0. 01 ）. Condusion Hyperoxia can induce the increase of lung heme oxygenase-1 expression in rat pups.

关 键 词：高浓度氧 血红素加氧酶-1 肺损伤 未成年大鼠 

分 类 号：R363[医药卫生—病理学]