熊果酸对胃癌细胞中cofilin-1蛋白表达的影响  

Effect of ursolic acid on the expression of cofilin-1 in human gastric cancer cell

作　　者：季青[1] 赵晓艳[1] 胡玉娜[1] 张隆[1] 倪振华[1] 康向东[1] 

机构地区：[1]上海中医药大学附属普陀医院检验科,上海200062

出　　处：《基础医学与临床》2011年第2期192-195,共4页Basic and Clinical Medicine

摘　　要：目的研究熊果酸作用胃癌细胞BGC-823后丝切蛋白cofilin-1表达的变化。方法熊果酸作用于细胞BGC-823,双向电泳结合质谱检测胞质内cofilin-1的表达,免疫印迹检测cofilin-1蛋白和细胞色素C的表达。结果胞质内cofilin-1蛋白表达降低(P<0.01),而线粒体表达却明显升高(P<0.01),同时细胞色素C在胞质中的表达量也明显下调(P<0.01)。结论熊果酸作用BGC-823,可能使cofilin-1蛋白从胞质转位至线粒体内,促使细胞色素C释放,激活caspase级联反应,诱导细胞凋亡。Objective To investigate expression changes of cofilin-1 in cell BGC-823 treated with ursolic acid. Methods BGC-823 cell was treated with ursohe acid, expression changes of eofilin-1 was detected by 2-D-electrophoresis and mass spectrum, protein expression of cofilin-1 and cytochrome C was detected by Western blot. Results Lower expression of cofilin-1 in eytoplasm（P 〈0. 01 ） , but obvious raise in mitochondria （ P 〈 0. 01 ）, and down- regulation of cytochrome C in eytoplasm（P 〈 0. 01 ） were found. Conclusion BGC-823 treated with ursolic acid may transfer cofilin-1 transposition from cytoplasm to mitochondria, which promoted eytochrome C released from mitochondria, activates caspase cascade, and induces cell apoptosis.

关 键 词：熊果酸 双向电泳 丝切蛋白 细胞色素C 免疫印迹 

分 类 号：R735.2[医药卫生—肿瘤]