液相色谱分离-氢化物原子吸收测定血清中不同形态的有机砷化合物  

Speciation of Organic Arsenic Species by Using Liquid Chromatography Coupled with Hydride-Generation Atomic Absorption Spectrometry

作　　者：欧阳津[1] 时彦[1] 张新荣[2] 

机构地区：[1]北京师范大学化学系,北京100875 [2]清华大学化学系,北京100084

出　　处：《分析化学》1999年第10期1151-1155,共5页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种色谱分离-紫外线消解-氢化物原子吸收光谱测定6种有机砷化合物的方法.这6种砷化合物分别是:甲基胂酸(MMA)、二甲基胂酸(DMA)、胂三甲胺内酯(AsB)、胂胆碱(AsC)、三甲基氧化胂(TMAO)和四甲基砷(TMAs^+).由于AsB、AsC和TMAs^+不能直接与NaBH_4反应产生氢化物,因此在色谱柱与检测器之间增设了一个紫外线消解装置,使这些砷化合物在发生氢化物前先行消解.本法适用于分析血清中的有机砷化合物.从进样到获得色谱图的过程约需10min.检出限(μg/L)为MMA(1.0);DMA(1.3);AsB(1.5);AsC(1.5);TMAO(2.0)和TMAs^+(1.6).A hyphenated technique was developed for the speciation of six organic arsenic species including monomethylarsonic acid ( MMA ) , dimethylarsinic acid ( DMA ) , arsenobetaine ( AsB ) , arsenocholine (AsC), trimethylarsinic oxide (TMAO) and tetramethylaisonium ion (TMAs+ ) by using high performance liquid chromatography coupled to hydride generation atomic absorption spectrometry with on-line sample digested by ultraviolet photo-oxidation process. The liquid chromatographic separation, photo-oxidation, hydride generadon and atomic absorption spectrometric measurements could be completedon-line within 10 min. The detection limits were 1.0, 1.3, 1.5, 1.5, 2.0 and 1.6μg/L for MMA,DMA, AsB, AsC, TMAO and TMAs+ , respectively. The developed method could be applied to thedetermination of arsenic species in serum of uraemic and dialysis patients.

关 键 词：形态分析 有机砷化合物 血清 砷 原子吸收光谱 

分 类 号：R446.112[医药卫生—诊断学] O627.52[医药卫生—临床医学]