强抗锌鞘氨醇单胞菌的分离鉴定及其抗性机理  

Isolation,Identification and Resistance Mechanism of a Zn-Resisting Bacterium,Sphingomonas sp.DX-T3-03

作　　者：王慧萍[1] 谢学辉[1] 柳建设[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《东华大学学报（自然科学版）》2010年第6期674-679,共6页Journal of Donghua University(Natural Science)

基　　金：全国优秀博士学位论文作者专项资金资助项目(200549);国家自然科学基金资助项目(50874032);上海市重点学科建设资助项目(B604)

摘　　要：从江西德兴铜矿重金属污染土壤中筛选得到一株对重金属锌具有极强抗性的菌株,命名为DX-T3-03.对该菌株进行形态和生理生化实验,并结合16SrDNA序列分析,鉴定其归属为鞘氨醇单胞菌属(Sphingomonas sp.).该菌对Zn2+的抗性高达3600mg/L,具有很高的应用价值.通过红外光谱和SEM-EDX(Scanning Electron Microscope-Energy Dispersive X-ray spectrometry)分析对其吸附机理做了初步探索,结果表明:DX-T3-03的细胞壁上参与积累作用的化学官能团主要有-OH,-NH,-COOH,-PO3-4等;高浓度锌在细菌细胞表面形成沉淀而达到对锌的富集与解毒.A Zn-resisting bacterium with high concentration,named DX-T3-03,was isolated from the metal-polluted sediment of soils around Dexing copper mine.Based on 16S rDNA sequence analysis,and its morphological and physiological characteristics,DX-T3-03 was identified as Sphingomonas sp..Minimal inhibitory concentration of Zn2＋ for this bacterium was 3 600 mg/L higher than others.The absorption mechanism of Zn2＋ was studied by FT-IR（Fourier Transform Infrared spectorscopy）and SEM-EDX（Scanning Electron Microscope-Energy Dispersive X-ray spectrometry）analysis.The results showed that the possible functional groups participated during the accumulation of Zn2＋ were —OH,—NH,—COOH,—PO3-4,and high concentration of zinc precipitated in the bacterial cell surface to achieve accumulation and detoxification.

关 键 词：重金属 鞘氨醇单胞菌 分离鉴定 生物吸附 

分 类 号：X172[环境科学与工程—环境科学]