端粒酶核酶抑制裸鼠移植瘤生长的实验研究  

Study on the inhibition of nude mice transplantation tumor growth by telomerase ribozyme

作　　者：屈艺[1] 刘菽秋[1] 张朝良 白绍槐[1] 欧阳雪松[1] 刘柏林[1] 

机构地区：[1]华西医科大学分子生物研究室,成都610041

出　　处：《中华医学遗传学杂志》1999年第6期368-370,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金资助;CMB基金资助

摘　　要：目的　探讨端粒酶核酶对裸鼠移植瘤生长的影响。方法　构建了人子宫颈癌细胞株HeLa 裸鼠移植瘤,将不同剂量端粒酶核酶真核表达质粒pXJ-neo-teloRZ用脂质体包裹后进行瘤体内多点注射,对照组注射生理盐水或空质粒载体。连续注射14 天,测量肿瘤体积和重量,检测瘤组织端粒酶活性,并作病理切片检查。结果　端粒酶核酶使肿瘤组织端粒酶活性下降,肿瘤组织凋亡增加,明显地抑制了裸鼠移植瘤生长。结论　该端粒酶核酶可望成为有效的端粒酶抑制剂,在肿瘤基因治疗中发挥作用。Objective To explore whether telomerase ribozyme could inhibit the growth of nude mice transplantation tumor.Mehtods A xenograft human nude mouse model was constructed. A total of 24 mice were divided into 4 groups, namely, the saline control(Group 1), the blank plasmid control(Group 2), the 20μg/mouse/day p XJ neo teloRZ (Group 3) and the 30μg/mouse/day p XJ neo teloRZ (Group 4).All plasmids were packaged by lipofectamine and used for subcutaneous, continuous infusions over 14 days. Tumor measurements and observations of animal behavior were recorded daily. On completion of the study, the mice were killed by cervical dislocation and their organs were removed, weighed, and stored in formaldehyde for histological examination. The telomerase activities of tumor tissues of each group were also detected.Results The telomerase ribozyme effectively inhibited the telomerase activities of tumor tissues and promoted the apoptosis of tumor cells. The in vivo studies showed a significant decrease in tumor size in mice treated with p XJ neo teloRZ when compared to the mice treated with controls.Furthermore, the in vivo effect of p XJ neo teloRZ was dose dependent.Conclusion Telomerase ribozyme is a powerful telomerase inhibition agent; probably it could be effective in tumor gene therapy.

关 键 词：端粒酶 核酶 移植瘤 肿瘤细胞凋亡 

分 类 号：R73－3[医药卫生—肿瘤]