脓毒血症时大鼠心血管系统血红素加氧酶的变化  

Alterations of heme oxygenase in cardiovascular system during sepsis

作　　者：李玉明[1] 刘俊昌[1] 张敏[1] 郑新程[1] 吴立玲[1] 时安云[1] 伍贻经[1] 

机构地区：[1]北京医科大学病理生理学教研室,北京100083

出　　处：《北京医科大学学报》1999年第6期497-500,共4页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：探讨脓毒血症时心血管系统血红素加氧酶（ｈｅｍｅ ｏｘｙｇｅｎａｓｅ，ＨＯ）的变化及作用。方法：盲肠结扎穿孔法复制大鼠腹膜炎脓毒血症模型，术后９ ｈ 和１８ ｈ 测血流动力学指标及血浆乳酸脱氢酶（ｌａｃｔａｔｅ ｄｅｈｙｄｒｏｇｅｎａｓｅ，ＬＤＨ），取心、主动脉测ＨＯ 活性和丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ） 含量，Ｎｏｒｔｈｅｒｎ Ｂｌｏｔ 测ＨＯ１ｍＲＮＡ 表达。结果：早期组血流动力学指标及血浆ＬＤＨ 无明显变化，心脏与主动脉ＭＤＡ 含量分别增加６０．３ ％ 和９６ ．５ ％ （Ｐ＜ ０ ．０５） ，心脏ＨＯ 活性比对照组升高７２ ．１％ （Ｐ＜ ０．０１） ，主动脉ＨＯ 活性无明显变化。晚期组血压降低、心功能下降，血浆ＬＤＨ与组织ＭＤＡ含量升高，心脏ＨＯ 活性仍维持高水平，主动脉ＨＯ 活性比早期组增加２６１ ％ （Ｐ＜ ０．０１）。心脏ＨＯ１ ｍ ＲＮＡ表达与ＨＯ 活性变化趋势相同；早、晚期主动脉ＨＯ１ｍＲＮＡ表达分别增加４９ ．２％ （Ｐ＜ ０．０５） 和１４３％（ Ｐ＜０ ．０１） 。结论：脓毒血症时心脏与主动脉ＨＯ 活性及ＨＯ１ ｍＲＮＡ 表达增加，但变化趋势不同；Objective: To study the alterations of heme oxygenase(HO) in cardiovascular system during sepsis. Methods: A rat sepsis model was induced by cecal ligation and puncture. In early and late sepsis, mean blood pressure(MBP),left ventricular function parameters and blood plasma lactate dehydrogenase (LDH) were measured. Then HO activities and MDA contents of the heart and aorta were examined. HO 1 mRNA expression was examined by Northern Blot. Results: In early sepsis, HO activity in the heart increased by 72.1% ( P <0.01)compared with control;while HO activity in the aorta, MBP, left ventricular function and blood plasma LDH had no significant changes. MDA contents in the heart and aorta increased by 60.3% and 96.5% respectively( P <0.05).In late sepsis,HO activity in the heart was still maintained at the high level while that in the aorta increased by 261% ( P <0.01)compared with that in early sepsis MBP,left ventricular function decreased and blood plasma LDH and MDA contents in the heart and aorta increased significantly ( P <0.01).HO 1 mRNA expression in the heart was the same as the alteration tendency of HO activity but that in the aorta was increased by 49.2% ( P <0.05) and 143% ( P <0.01) respectively in early and late sepsis. Conclusion: HO activity and HO 1 mRNA expression in the heart and aorta are increased during sepsis and the inducable HO plays an important role during sepsis.In early sepsis HO can protect organs from injury but in late sepsis it may be one of the most important reasons for hypotension.

关 键 词：毒血症 酶学 血红素氧化酶 心血管系统 

分 类 号：R631.1[医药卫生—外科学]