声动力化学疗法促大鼠脑胶质瘤细胞凋亡及抗血管作用的实验研究  

The apoptosis and anti-vascular effects of sonodynamic chemistry therapy on glioma cells

作　　者：宋大勇[1] 岳武[2] 魏盾[1] 林述凯[1] 孙超[1] 李建华[2] 

机构地区：[1]北华大学附属医院神经外一科,吉林吉林132011 [2]哈尔滨医科大学第四临床医学院神经外科,黑龙江哈尔滨150001

出　　处：《中风与神经疾病杂志》2010年第12期1082-1085,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(30970834);黑龙江省卫生厅课题(2006-206);黑龙江省卫生厅课题(2007-408)

摘　　要：目的探讨声动力化学疗法(SDT)对大鼠脑胶质瘤细胞凋亡及微血管蛋白表达的关系。方法以大鼠颅内C6胶质瘤为实验模型,观察4组(SDT、US、HMME、对照)处理后脑胶质瘤原位细胞凋亡、Cyt-C蛋白、Caspase-3蛋白表达水平。结果原位凋亡检查见HMME组和对照组凋亡细胞极少,各时间点凋亡率无明显差别。处理后24h,US组凋亡率高于HMME组和对照组;处理后3d、7d,凋亡率与HMME组和对照组无明显差别。SDT组处理后24h,凋亡率明显高于其它3组。处理后3d、7d,凋亡率与其它3组无明显差别。HMME组和对照组各时间点MVD密度、VEGF蛋白表达无明显差别。US组处理后24h,MVD密度、VEGF蛋白表达与HMME组和对照组比较,略下降;处理后3d、7d,2项表达结果与HMME组和对照组无明显差别。SDT组处理后24h MVD密度、VEGF蛋白表达较其它3组明显下降;处理后3d,二者有所升高,但仍较其它3组低;处理后7d,VEGF表达结果与其它3组无明显差别。结论 SDT处理后早期可通过血管靶向作用促进体内胶质瘤细胞凋亡。Objective To investigate the effect of SDT on apoptosis and microvascular protein expression of C6 glioma cells.Methods We took intracranial C6 rat glioma as experimental model.After dealing with intracranial glioma,we observed the four groups（sonodynamic therapy（SDT）,Ultrasound（US）,hematoporphyrin monomethyl ether（HMME）,control） in situ apoptosis,Cyt-C protein and Caspase-3 protein expression of glioma.Results We checked in situ apoptosis and found control and HMME groups were few apoptotic cells.Each time point was no significant difference in apoptotic rate.The apoptotic rate of the US group was higher than HMME and control groups after 24h.The apoptosis rate of HMME group and the control group was no significant difference after 3d and 7d.The apoptotic rate of SDT group was significantly higher than the other three groups after 24h.The apoptotic rate of SDT group and the other three groups was no significant difference after 3d and 7d.MVD density and VEGF expression of HMME group and the control group was no significant difference at each time point.MVD density and VEGF protein expression of HMME group slightly decreased than the control group after 24h.Expression of the two results in HMME group and the control group was no significant difference.The MVD density and VEGF protein expression of SDT group was significantly lower than the other three groups.The MVD density and VEGF protein expression had increased,but still lower than the other groups after 3d.VEGF expression of SDT group and the other three groups was no significant difference.Conclusion SDT treatment can promote apoptosis of glioma by vascular targeting effect in vivo in early stage.

关 键 词：神经胶质瘤 声动力疗法 血卟啉类 细胞凋亡 血管靶向 

分 类 号：R739.4[医药卫生—肿瘤]