核不均一性核糖核蛋白A2/B1在脑缺血再灌注大鼠脑皮质分布的变化  

Changes of Translocation of Heterogeneous Nuclear Ribonulcleoprotein A2/B1 of Brain Cortex after Cerebral Ischemic Reperfusion in Rats

作　　者：刘一沉[1,2] 李保全[2] 张效东[2] 孟莲花[2] 白侠[2] 张成岗[3] 王航雁[1] 

机构地区：[1]中国人民解放军总医院儿内科,北京100853 [2]中国人民解放军第159医院儿内科,河南驻马店463000 [3]军事医学科学院放射与辐射研究所医学神经生物学国家重点实验室,北京100850

出　　处：《实用儿科临床杂志》2010年第24期1862-1864,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30973107)

摘　　要：目的研究大鼠脑缺血再灌注损伤后核不均一性核糖核蛋白(hnRNP)A2/B1在大鼠脑皮质表达位置变异与再灌注损伤时间的关系。方法随机选取30只雄性SD大鼠,分为假手术组、缺血2h再灌注3h、6h、12h、24h、48h6组,每组各5只。线栓法制作稳定的大鼠右侧大脑中动脉栓塞?再灌注实验模型,采用免疫组织化学和Nissl染色法测定再灌注损伤后3h、6h、12h、24h、48h缺血侧扣带回、纹状皮质、颞叶皮质、梨状皮质区域大脑皮质hnRNPA2/B1表达分布位置的改变,并与假手术组进行比较。同时观察缺血再灌注损伤大鼠脑皮质病理学改变。结果 1.假手术组中hnRNPA2/B1蛋白主要定位于神经元细胞核中,缺血再灌注各组与假手术组相比,缺血再灌注后3h、6h、12h、24h、48h,hnRNPA2/B1出现核突易位的变异,并呈现先上升后下降的趋势。脑缺血再灌注3h后,hnRNPA2/B1表达区神经元出现核突易位(P<0.01),12h后hnRNPA2/B1易位神经元数目持续增加(P<0.01),24h达高峰(P<0.01);再灌注48h后,hnRNPA2/B1表达区神经元核浆、核突易位降至0。2.与假手术组比较,缺血再灌注各组脑皮质损伤严重,尤其缺血再灌注24h组神经细胞变性坏死显著。结论 hnRNPA2/B1可能在基因转录后水平参与调控缺血再灌注脑损伤。Objective To investigate the dynamic changes of translocation of heterogeneous nuclear ribonulcleoprotein A2/B1（hnRNP A2/B1） in rats after brain cortex and its relation to the time of reperfusion.Methods A total of 30 male SD rats were randomly divided into 6 groups：sham operated group without ischemia（n=5） and other 5 groups with 2 h ischemic followed by reperfusion at different times（3 h,6 h,12 h,24 h,48 h;n=5 in each group）.Focal cerebral ischemia reperfusion models were induced by using the right middle cerebral artery（MCA） of SD rats and occluded for 2 h by the use of an intralaminal filament and recirculation was instituted for 3 h,6 h,12 h,24 h,48 h.Sham operated rats were not subjected to middle cerebral artery occlusion/reperfusion served as controls.Immunohistochemistry technique was employed to examine localization changes in hnRNP A2/B1 in rat cerebral cortex（including cingulum cortex,striate cortex,temporal cortex and piriform cortex） after ischemical reperfusion injury.Morphological changes in neuronal cells were identified in cerebral cortex after ischemic reperfusion injury by Nissl′s staining method.Results 1.Immunohistochemistry observations demonstrated a nucleoplasmic localization of hnRNP A2/B1 in cerebral cortex in sham operated group.After 3 h,6 h,12 h,24 h,48 h of ischemia reperfusion in cerebral cortex,hnRNP A2/B1 were observed to translocate from nuclear to cytoplasm and neuronal dendrite,which rose first and then fell.Compared with the sham operated group,translocation of hnRNP A2/B1 from nucleus to cytoplasm and even to neurons was observed in cerebral cortex at 3 h of ischemic reperfusion（P0.01） and continuously increased at 12 h（P0.01）,reached a peak at 24 h（P0.01） of reperfusion.After 48 h after ischemic reperfusion,the number of hnRNP A2/B1 translocation neurons reduced dramatically to the minimum level.2.With contrast to the sham operated group,neuronal damage was relatively severe in the cerebral cortex in ischemic reperfusion groups.After 24 h

关 键 词：核不均一性核糖核蛋白A2/B1 细胞核-细胞质移位 缺血再灌注损伤 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]