小鼠全脑缺血再灌注模型的制备及评价  

Establishment and evaluation of the global cerebral ischemia-reperfusion model in mice

作　　者：陈远寿[1] 陈旻[2] 张弛[2] 

机构地区：[1]遵义医学院生理教研室,贵州遵义563003 [2]中国科学院神经科学研究所

出　　处：《中国老年学杂志》2011年第3期435-438,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.30721004)

摘　　要：目的建立与评价一种小鼠全脑缺血再灌注模型。方法采用三动脉阻塞(3-VO)的方法制备C57BL/6小鼠全脑缺血模型;分别应用F-JB染色检测海马神经元变性死亡情况和尼氏染色检测海马神经元存活情况。结果①C57BL/6小鼠全脑缺血8min再灌注3d,在海马CA1区有神经元死亡,缺血12和16min后海马CA1、CA3、DG区神经元存活的数量明显少于假手术对照组(P<0.01);②与假手术对照组比较,C57BL/6小鼠全脑缺血12min再灌注3、7、28 d后CA1、CA3、DG区存活的神经元数量显著减少(P<0.01);③缺血12 min再灌注3d后,在C57BL/6小鼠大脑皮层、丘脑、纹状体均有神经元死亡。结论 3-VO小鼠全脑缺血模型是用于研究局部脑缺血再灌注损伤较为理想的动物模型。Objective To establish and evaluate the global cerebral ischemia-reperfusion（I-R） model in C57BL/6 mice.Methods Male C57BL/6 mice were subjected to global ischemia by the three-vessel occlusion（3-VO） method.Hippocampal sections were processed for Fluoro-Jade B to detect degenerating neurons and Nissl staining to identify surviving neurons.Results ①There was CA1 hippocampal neuronal degeneration in C57BL/6 mice at 8 min ischemia after reperfusion 4 d.The CA1,CA3 and dentate granule surviving neurons significantly reduced after 12 and 16 min ischemia compared with those of the sham group（P0.01）.②Compared with those of sham group,the CA1,CA3 and dentate granule surviving neurons count were significantly reduced at 12 min ischemia after reperfusion 3,7 and 28 d（P0.01）.③Neuronal degeneration of the cortex,striatum and thalamus were also observed at 12 min ischemia after reperfusion 3 d.Conclusions The 3-VO global cerebral I-R mice model is an ideal experimental animal model for subsequent study.

关 键 词：全脑缺血模型 海马 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]