G-CSF干预对老龄慢性脑缺血大鼠模型认知改善与胶质细胞可塑性的影响  

The correlation of cognitive handicap and gliacyte plasticity after G-CSF treatment in chronic cerebral hypoperfusion of the aged rats

作　　者：舒细记[1] 柳威[1] 周红艳[1] 陈莹[1] 陈艳华[1] 熊巍鹏[1] 艾永循[1] 

机构地区：[1]江汉大学医学院病理学与病理生理学教研室,湖北武汉430056

出　　处：《中国老年学杂志》2011年第3期450-452,共3页Chinese Journal of Gerontology

基　　金：湖北省自然科学基金资助项目(No.2006AB190)

摘　　要：目的探讨粒细胞集落刺激因子(G-CSF)干预后,慢性脑缺血老龄鼠学习记忆功能改善与海马胶质细胞可塑性改变的相关性。方法 12月龄雄性SD大鼠2VO术后饲养3个月,构建15月龄的2VO慢性脑缺血老龄鼠模型。分为2VO/G-CSF组(n=10)、2VO/NS组(n=7),Sham组(n=10)。采用Morris水迷宫检测大鼠空间学习记忆能力,通过免疫组化和图像分析技术检测海马CA1区胶质细胞的数目及胞质突起长度。结果水迷宫实验:2VO/NS组逃逸时间显著长于Sham组和2VO/G-CSF组(P<0.01);2VO/NS组大鼠在平台象限的停留时间少于Sham组(P<0.01)和2VO/G-CSF组(P<0.05);2VO/NS组大鼠跨过平台区域的次数少于Sham组和2VO/G-CSF组(P<0.01)。HE染色发现G-CSF干预后海马CA1区锥体细胞排列层数增加,胞体增大。免疫组化染色:2VO/NS组海马CA1区GFAP阳性细胞少于Sham组和2VO/G-CSF组(P<0.05)。2VO/NS组大鼠GFAP阳性细胞胞质突起长度低于Sham组和2VO/G-CSF组(P<0.05)。认知功能与胶质细胞可塑性的相关分析:2VO/NS组、Sham组及2VO/G-CSF组海马CA1区胶质细胞的数量、胞质突起长度与空间记忆能力均呈正相关(P<0.05)。结论慢性脑缺血可致老龄鼠的空间学习记忆能力明显受损,G-CSF可诱导海马锥体细胞增生及胶质细胞可塑性改变,有效改善空间学习记忆功能。其中,胶质细胞可塑性改变与空间记忆功能改善密切相关。Objective To investigate correlation of granulocyte colony stimulating factor（G-CSF） on cognitive handicap and gliacyte plasticity in chronic cerebral ischemia aged rats.Methods 12 months male SD rats were regularly fed for 3 months after bilateral common carotid arteries occlusion surgery（2VO） to set up 15 month-old chronic cerebral ischemia aged rats.The male SD rats were randomly divided into 2VO/NS（n=7）,Sham（n=10） and 2VO/G-CSF（n=10） groups.Spatial learning and memory were accessed by Morris water maze,the number and cytoplasmic process length of GFAP positive cells in hippocampal CA1 region were analyzed by immunohistochemistry and image analysis.Results Escape latency of 2VO/NS rats was longer than that of Sham and 2VO/G-CSF rats（P0.01）.2VO/NS rats spent less time in the platform quadrant than Sham（P0.01） and 2VO/G-CSF rats（P0.05）. As for number of crossing the platform area,2VO/NS rats was less than Sham and 2VO/G-CSF rats（P0.01）.Pyramidal cell arrayed sparser and more indiscriminate in 2VO/NS rats compared to that of Sham and 2VO/G-CSF groups.The number of GFAP positive cells of 2VO/NS group was significantly less than that of Sham and 2VO/G-CSF groups（P0.05）.The cytoplasmic process length of GFAP positive cell in 2VO/NS group was significantly less than that of Sham and 2VO/G-CSF groups（P0.05）.Number and the cytoplasmic process length of glia cells were positively related to spatial learning among 2VO/NS,Sham and 2VO/G-CSF groups.Conclusions Chronic cerebral hypoperfusion impairs spatial learning and memory in aged rats. G-CSF can enhance cognitive function by promoting pyramidal cell hyperplasia and alteration of gliacyte plasticity.Simultaneously,the alteration of gliacyte plasticity is related to improvement of spatial learning after continuously cerebral hypoperfusion.

关 键 词：慢性脑缺血 粒细胞集落刺激因子 学习记忆 胶质细胞可塑性 老龄鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]