组织蛋白酶B在β-淀粉样蛋白刺激星形胶质细胞炎性反应中的作用  

Effect of Cathepsin B on Inflammatory Reaction Induced by Activated Astrocyte of β-Amyloid

作　　者：姚丽芬[1] 王振峰[1] 杨颖[1] 许芳[1] 赵广健[1] 徐春晶[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,黑龙江哈尔滨150001

出　　处：《航空航天医药》2010年第12期2131-2133,共3页Aerospace Medicine

基　　金：黑龙江省教育厅基金项目(11521134)

摘　　要：目的：研究组织蛋白酶B（Cathepsin B,CB）与β-淀粉样蛋白（β-amyloid protein,Aβ）激活星形胶质细胞诱导炎性反应的关系。方法：应用免疫细胞化学方法鉴定体外培养的星形胶质细胞;四唑盐法检测细胞活性,加入不同浓度溶解状态的Aβ1-40后观察细胞形态学改变,western blot法测定细胞上清CB水平。结果：在5~60μmol/L溶解状态的Aβ1-40作用24 h的条件下,星形胶质细胞的活性没有受到影响,其细胞形态无明显变化,但是随着时间的延长星形胶质细胞逐渐出现肿胀、坏死。40~60μmol/L的溶解状态Aβ1-40诱导的星形胶质细胞上清中检测到CB。结论：Aβ1-40可激活星形胶质细胞,诱导其释放CB,提示CB的神经毒性作用可能是通过诱导神经细胞凋亡实现的。Objective：To investigate the relationship between β-amyloid（Aβ） and astrocyte secretion.Methods：Cultured astrocytes were treated with Aβ1-40 in different concentrations,then the cell morphology and cathepsin B（CB） secretion were observed by microscopy and western blot,respectively.The survival rate of astrocytes was assayed by MTT assay.Results：On the doses study,Aβ1-40 had no effects on astrocytes which did not change their morphology after treatment with Aβ1-40.With the time prolonged,the astrocytes appeared swollen in the medium and some became necrotic.The expression of CB was detected by western blot.Conclusions：Aβ1-40 activates and induces astrocytes releasing CB,which indicates the importance of the procession of Aβ accumulation related to the pathogenesis in AD mediated by astrocytes.

关 键 词：组织蛋白酶B 淀粉样Β蛋白 星形胶质细胞 阿尔茨海默病 

分 类 号：R741[医药卫生—神经病学与精神病学]