PDTC对LPS刺激的人牙周韧带细胞的作用研究  

Effects of pyrrolidine dithioearbamate on lipopolysaccharide-stimulated human periodontal ligament cells

作　　者：王淑霞 刘大勇[2] 刘南[2] 刘士有[2] 

机构地区：[1]北京市朝阳区太阳宫社区卫生服务中心口腔科,100028 [2]天津医科大学口腔医院

出　　处：《实用口腔医学杂志》2011年第1期50-53,共4页Journal of Practical Stomatology

摘　　要：目的:探讨核转录因子(nuclear factor kappa B,NF-κB)抑制剂吡咯烷二硫代氨基甲酸盐(pyrrolidine dithioearba-mate,PDTC)对脂多糖(lipopolysaccharide,LPS)刺激的人牙周韧带细胞的干预作用。方法:采用组织块法分离培养人牙周韧带细胞(human periodontal ligament cells,HPDLCs)。实验分为对照组与实验组,对照组细胞以含10μg/ml LPS的培养基培养;实验组分别以PDTC10、100、1 000μmol/L预处理细胞30 min后,加入含相同浓度LPS的培养基培养。ELISA检测细胞上清液中炎性细胞因子IL-1β、IL-8分泌量的改变。免疫荧光检测干预前后细胞内NF-κB的核转移改变。结果:PDTC抑制HPDLCs增殖,10μmol/L PDTC预处理对HPDLCs分泌IL-1β和IL-8无影响,其余2种浓度均可抑制IL-1β和IL-8的分泌,1 000μmol/L PDTC的抑制作用更强,与其它各组相比差异具有显著性(P<0.05)。免疫荧光显示PDTC预处理后细胞核NF-κB的表达明显减弱或消失。结论:PDTC可以抑制LPS刺激的HPDLCs分泌IL-1β与IL-8和细胞内NF-κB的核转移,说明NF-κB信号通路在牙周病的发生过程中具有重要作用,阻断该通路可能成为牙周病药物治疗的一种新策略。Objective：To study the effects of pyrrolidine dithioearbamate（PDTC）,a NF-κB inhibitor,on lipopolysaccharide（LPS）-stimulated human periodontal ligament cells（HPDLCs）.Methods：HPDLCs were prepared by using explant culture technique.Cells were devided into two groups.Control group was treated with culture medium containing 10 μg/ml LPS.Experimental group was incubated with 10,100 and 1 000 μmol/L of PDTC for 30 min respectively before culture medium containing 10 μg/ml LPS was added.Then IL-1β and IL-8 secreted into the culture medium was determined by an enzyme-linked immunosorbent assay（ELISA）.Nuclear translocation of NF-κB in the cells was observed by indirect immunofluorescence.Results：PDTC suppressed HPDLCs proliferation.10 μmol/ml PDTC didn＇ t inhibit the secretion of IL-1β and IL-8,but 100 μmol/L and 1 000 μmol/L PDTC did.And,more pronounced inhibition of 1 000 μmol/L PDTC showed that there was significant difference compared with the others（P0.05）.The NF-κB antibody staining showed strong green fluorescence signals in the nuclei after LPS stimulated,but PDTC treatment strongly reduced the intensity of nuclear staining.Conclusion：IL-1β and IL-8 expression and nuclear translocation of NF-κB in LPS-stimulated HPDLCs can be inhibited by PDTC.NF-κB signaling pathway play a role in the development of periodontal disease.

关 键 词：吡咯烷二硫代氨基甲酸盐 脂多糖 人牙周韧带细胞 核转录因子-ΚB 

分 类 号：R781.4[医药卫生—口腔医学]