沙利度胺对裸鼠卵巢癌皮下种植瘤微血管密度和细胞凋亡影响的研究  

Study of thalidomide on the microvessel density and apoptosis index of ovary cancer SKOV-3 subcutaneous implanted in nude mice

作　　者：杜娟[1] 黄学惠[1] 

机构地区：[1]昆明医学院第一附属医院妇科,昆明650032

出　　处：《现代妇产科进展》2011年第1期50-53,共4页Progress in Obstetrics and Gynecology

基　　金：云南省教育厅资助课题(No:08c0103)

摘　　要：目的:探讨沙利度胺(反应停,酞咪哌啶酮,thalidomide)对卵巢癌裸鼠皮下种植瘤的微血管密度和细胞凋亡的影响。方法:随机将20只卵巢癌裸鼠荷瘤模型分为4组,每组5只。(1)沙利度胺组:200mg.kg-1.d-10.2ml腹腔内注射(ip);(2)顺铂组:2mg.kg-1.d-10.2mlip;(3)联用组:顺铂2mg.kg-1.d-1,沙利度胺200mg.kg-1.d-1各0.2mlip;(4)生理盐水组:生理盐水0.2mlip。各组皮下种植瘤的瘤体直径平均为0.8cm时给药,给药2周,每周2次。给药2周后处死动物,检测各组肿瘤组织中微血管密度(MVD)及细胞凋亡指数(AI)。结果:(1)4组均未发现明显不良反应,沙利度胺组、联用组、顺铂组抑瘤率分别为57.47%、71.37%、42.18%,联用组抑瘤作用最显著(P<0.05);(2)与生理盐水组相比,沙利度胺组、联用组MVD受到明显抑制(P<0.05),顺铂组MVD无显著差异(P>0.05);(3)沙利度胺组、联用组、顺铂组AI均增高(P<0.05),且以联用组抑制作用最显著(P<0.05)。结论:腹腔注射沙利度胺可通过抑制肿瘤血管生成,诱导癌细胞凋亡,与化疗药物顺铂联用,有增强癌细胞凋亡的作用。Objective：To study the effect of thalidomide on microvessel density（MVD） and apoptosis of ovarian cancer cell subcutaneous implanted tumor.Methods：Ovarian cancer cells SKOV-3 were subtaneous implanted in nude mice and 4 groups were set up,n=5：（1） thalidomide group：200mg.kg-1.d-1,0.2ml,intraperitoneal injection（ip）.（2）Cisplatin（DDP）group：2mg.kg-1.d-1,0.2ml,ip.（3）Combined group：both thalidomide and DDP 0.2ml,ip.（4）Control group：saline 0.2ml,ip.When the mean tumor diameter reach ed 0.8 cm（the MTD 0.8cm）,each mouse was given twice a week,a total of 2 weeks.Animals were killed 2 weeks after administration,MVD and apoptosis index（AI） were detected in tumors tissue.Results：（1）4 groups were not found significant adverse effects.Tumor inhibition rates of the thalidomide group,combined group and DDP group were 57.47%,71.37%,42.18%.Combined group is the most significant tumor inhibition（P0.05）.（2）Compared with the control group,the thalidomide group,combined group of MVD were significantly inhibited（P0.05）.DDP group MVD was no significant difference（P0.05）.（3）Thalidomide group,combination group and DDP group were significantly higher in AI（P0.05）.The combined group is the most significant inhibition（P0.05）.Conclusion：Thalidomide can induce apoptosis in ovarian cancer by inhibiting tumor angiogenesis.The effect will also be increased by combination with the traditional chemotherapy drugs DDP.

关 键 词：沙利度胺 卵巢肿瘤 微血管密度 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]