沙苑子黄酮对人乳腺癌细胞MCF-7增殖抑制、诱导凋亡及上调p53表达、下调NF-κB、c-Myc表达作用  

FAC inhibits proliferation,induces apoptosis of human breast cancer MCF-7,increases expression of p53 and decrease expression of c-Myc and NF-κB

作　　者：韦翠萍[1] 邱秀芹[2] 顾振纶[3] 

机构地区：[1]苏州卫生职业技术学院药理教研室,江苏苏州215009 [2]苏州卫生职业技术学院生化教研室,江苏苏州215009 [3]苏州大学药理教研室,江苏苏州215123

出　　处：《南京医科大学学报（自然科学版）》2010年第11期1556-1559,1563,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:研究沙苑子黄酮(flavonoids of Astragalws complanatus,FAC)抑制人乳腺癌细胞MCF-7增殖、诱导其凋亡以及FAC对核转录因子κB(nuclear factor-κB,NF-κB)、c-Myc、p53表达的影响。方法:以不同浓度FAC(0.0625、0.125、0.25、0.5mg/ml)处理MCF-7细胞12、24、48、72h后,MTT法测定细胞增殖情况;用流式细胞分析术检测FAC诱导凋亡作用;用荧光免疫法、Western blot法检测凋亡相关蛋白的表达。结果:MTT法显示,不同浓度FAC作用MCF-7细胞24、48、72h后对细胞增殖具有明显抑制作用,与阴性对照组相比差异具有显著性(P<0.05);抑瘤率与FAC剂量、作用时间呈依赖性。流式细胞分析显示,FAC能明显诱导MCF-7细胞凋亡,0.0625、0.125、0.25、0.5mg/ml FAC作用细胞36h后,凋亡率分别为6.5%、18.3%、49.6%和57.9%。荧光免疫法及Western blot法显示,FAC作用后MCF-7中c-Myc与NF-κB表达明显减弱;而p53表达显著增强,并向细胞核内聚集。结论:FAC对MCF-7具有抑制增殖和诱导凋亡作用;其作用可能与其下调NF-кB、c-Myc表达、上调p53表达有关。Objective：To research the effects of flavonoids of Astragalus complanatus（FAC） on proliferation,apoptosis and expressions of p53,c-My and NF-кB in human breast cancer MCF-7 cells.Methods：The inhibit rate of MCF-7 cells treated by different concentration of FAC was studied by MTT at different times.The apoptosis rate of MCF-7 cell was essayed by flow cytometry after treated with effective concentration of FAC and time points according to MTT.The expressions of p53,c-Myc and NF-кB were quantified by fluorescence immunoassay and Western blot.Results：According to MTT,0.0625,0.125,0,25,0.5 mg/ml of FAC treated for 24 h,48 h,72 h markedly inhibited proliferation of MCF-7 （P 0.05,vs control）.According to flow cytometry,MCF-7 was induced to apoptosis by FAC.After 72 h treatment with FAC（0.0625,0.125,0.25,0.5 mg /ml）,the apoptosis rates were 6.5%,18.3%,49.6% and 57.9%,respectively.The expression of p53 was up-regulated,while c-Myc and NF-κB were markedly reduced detected by fluorescence immunoassay and Western blot.Conclusion：FAC could inhibit proliferation and induce apoptosis of MCF-7.Its role may be related to decreased expression of NF-кB and c-Myc,increased expression of p53.

关 键 词：沙苑子黄酮 MCF-7 凋亡 NF-ΚB C-MYC p53 

分 类 号：R737.9[医药卫生—肿瘤]