H2O2在戊四氮致痫模型中对海马神经元兴奋毒性的影响  

Effect of H_2O_2 on the Excitotoxicity of Neurons in Rats with Seizures Induced by Pentylenetrazole

作　　者：孙蕾[1] 陈蕾[1] 尧青[2] 朱威[2] 李正 

机构地区：[1]华中科技大学同济医学院附属同济医院第二临床学院,05级临床医学八年制武汉430030 [2]华中科技大学同济医学院基础医学院解剖学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2010年第6期780-783,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究戊四氮致痫模型中,大鼠海马过量产生的H2O2对海马神经元兴奋毒性的影响。方法 SD雄性大鼠随机分为3组,每组14只。A组为生理盐水对照组,B组为戊四氮致痫组,C组为H2O2酶处理＋戊四氮致痫组。动物造模后,观察各组大鼠癫痫发作情况;然后分别采用分光光度法测定大鼠海马H2O2含量,免疫组织化学法（SABC法）检测大鼠海马谷氨酸（Glu）免疫反应性,RT-PCR法检测大鼠海马Caspase-3 mRNA水平。结果 A组大鼠无明显癫痫发作,B组大鼠有癫痫大发作达Ⅴ级,C组大鼠癫痫发作为Ⅱ~Ⅲ级。B组大鼠海马H2O2含量、Glu免疫反应性、Caspase-3 mRNA表达水平均显著高于A组（均P〈0.05）,C组海马H2O2、Glu免疫反应性、Caspase-3 mRNA表达水平均明显低于B组（均P〈0.05）。结论戊四氮诱发癫痫发作后,导致H2O2过量产生,其对海马神经元具有兴奋毒性作用。Objective To explore the effect of H2O2 on the excitotoxicity of neurons in rats with pentylenetrazole（PTZ）-induced seizures.Methods Rats were randomly divided into 3 groups：saline group,PTZ group and CAT＋PTZ group.The behaviors of the animals were observed for 2 h.The contents of H2O2 in hippocampus of rats were measured using spectrometry.The immunoreactions of glutamate in hippocampus of rats were examined by SABC method.The expression of Caspase-3 mRNA in hippocampus of rats was detected by using RT-PCR.Results Seizures were markedly attacked,and immunoreactions of Glu and expression of Caspase-3 mRNA were obviously increased in PTZ group as compared with control group（P0.05）.Seizures were greatly inhibited,and immunoreactions of Glu and expression of Caspase-3 mRNA were obviously decreased in PTZ＋CAT group as compared with PTZ group（P0.05）.Conclusion PTZ-induced seizure can produce excess H2O2,which can result in excitotoxicity to neurons in the hippocampus.

关 键 词：癫痫 H2O2 谷氨酸 CASPASE-3 兴奋毒性 

分 类 号：R742.1[医药卫生—神经病学与精神病学]