SO_2衍生物对C57BL/6小鼠肝脏T细胞亚群CD4＋/CD8＋的影响  

Effect of SO_2 Derivatives on Liver T Lymphocytes Subsets CD4+/CD8+ of C57BL/6 Mice

作　　者：武冬梅 李素清 狄晓艳 白剑英[2] 

机构地区：[1]山西大学黄土高原研究所,山西大学环境与资源学院,太原030006 [2]山西医科大学公共卫生学院,太原030001

出　　处：《生态毒理学报》2010年第3期351-355,共5页Asian Journal of Ecotoxicology

基　　金：山西省自然科学基金项目(No.2009011039-2)

摘　　要：为探讨二氧化硫(SO2)的肝脏免疫毒理效应,利用流式细胞仪(FACS)检测技术,分析了SO2体内代谢衍生物——亚硫酸钠(Na2SO3)与亚硫酸氢钠(NaHSO3)混合液(两者摩尔比为3:1)腹腔注射染毒对C57BL/6小鼠肝脏淋巴细胞T细胞亚群CD4+和CD8+的百分数以及CD4+/CD8+细胞比值的影响.实验组剂量分别为25、100、400mg·kg-1(body weight),染毒一周后,制备肝脏淋巴细胞悬液,经特异性荧光标记的CD4(FITC)、CD8(PE)单克隆抗体染色后,采用FACS流式细胞仪检测T淋巴细胞亚群百分数.研究发现:1)染毒后所有处理组肝脏CD4+T细胞所占的百分数显著升高(p<0.05);2)CD8+T细胞所占的百分数在100mg·kg-1、400mg·kg-1染毒组显著降低(p<0.05);3)CD4+/CD8+的比值在100mg·kg-1、400mg·kg-1染毒组显著升高(p<0.01).研究结果显示:SO2体内衍生物亚硫酸钠和亚硫酸氢钠可使肝脏CD4+/CD8+T细胞的比值显著升高,即SO2衍生物可使肝脏CD4和CD8淋巴细胞比例严重失调而使机体产生免疫紊乱.The objective of this study was to assess the effects of sulfur dioxide derivatives（sodium sulfite and sodium bisulfite,their molecular ratio was 3：1）on liver T lymphocyte subsets through detecting the percentages of CD4＋ and CD8＋T cells.Twenty-four adult male C57BL/6 mice were divided into four groups.Mice were given 25,100,400mg·kg-1（body weight） sulfur dioxide derivatives by intraperitoneal injection（ip） every day for 7 days.Mice in the control group were intraperitoneally injected with the same volume of saline.Single-cell suspensions of liver lymphocytes were prepared.After labeling with FITC conjugated rat-anti-mouse CD4（monoclonal） and PE conjugated rat-anti-mouse CD8 （monoclonal）,the phenotypes of liver T lymphocytes were analyzed by flow cytometry（FACS）.The results showed that the percentages of CD4＋T cells among all treatment groups were significantly increased（p0.05） compared with that of the control group.However,the percentages of CD8＋T cells in higher dose groups（100,400mg·kg-1） were significantly decreased（p0.05）.The ratio of CD4＋ and CD8＋T cells was also significantly increased in higher dose groups （p0.01）.This study indicates that sulfur dioxide derivatives could significantly increase the ratio of CD4＋ and CD8＋,inducing the immune system disorganized.

关 键 词：SO2衍生物 T淋巴细胞亚群 CD4+/CD8+ 肝脏 

分 类 号：X171.5[环境科学与工程—环境科学]