PPARα基因对安卡×固始鸡资源群胴体品质的遗传效应分析  

Genetic Effects of PPARα Gene on Carcass Traits in Anak × Gushi Chicken Resource Population

作　　者：田亚东[1] 亢娟娟[1] 孙桂荣[1] 韩瑞丽[1] 康相涛[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《华北农学报》2010年第6期14-18,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划(2006BDA01A09);河南省杰出人才创新基金(074200510009)

摘　　要：由793只安卡×固始鸡F2资源群为试验材料,采用PCR-SSCP技术和DNA测序的方法对PPARα基因的编码区进行SNP检测。结果发现,外显子4存在1个多态性位点,经限制性内切酶BspHⅠ酶切后显示出EE、EF、FF 3种基因型。利用SAS8.0软件分析不同基因型对胴体品质的影响,结果表明,PPARα基因NC_006088:T32311C的多态性显著影响鸡的腹脂重和腹脂率(P<0.05),而对屠体重、皮下脂肪厚、肌间脂肪带宽、半净膛重和全净膛重等无显著影响(P>0.05)。EE型和EF型的腹脂重和腹脂率显著高于FF型(P<0.05),而EE型和EF型之间无显著差异(P>0.05)。PPARα基因影响脂肪代谢,可以作为分子标记用于鸡脂肪性状的分子标记辅助选择。A Anak×Gush resource population including 793 F2 chickens was founded by F2 design to analyze the PPARα gene polymorphism by the PCR-SSCP combined with sequencing techniques.The single nucleotide polymorphism（SNP）were found in exon 4 regions of the PPARα.Three kinds of genotypes detected in the resource population were defined as EE,EF and FF by digesting with BspH I restriction endonuclease.Effects of different genotypes on carcass traits were evaluated with SAS8.0 software.Results showed that the polymorphism of loci NC_006088：T32311C in PPARα gene was significantly related to the abdominal fat weight（AFW）and abdominal fat percentage（AFP）（P 0.05）,and no significance with carcass weight,subcutaneous fat thickness,fat band width,semi-evisceration weight and evisceration weight（P 0.05）.The chickens with the genotype of EE and EF had the higher AFW and AFP than those of FF（P 0.05）,while there was no significant difference between the genotype of EE and EF（P 0.05）.Result implied that PPARα gene affecting lipometabolism was a good marker for using in marker associated selection program.

关 键 词：鸡 F2资源群 PPARα基因 多态性 胴体品质 

分 类 号：S858.03[农业科学—临床兽医学]