草莓乙烯受体FaEtr2基因植物表达载体构建  

Expression Vector Construction of Strawberry FaEtr2 Gene

作　　者：宋春丽[1] 赵丛枝[2] 马俊莲[2] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《华北农学报》2010年第6期71-73,共3页Acta Agriculturae Boreali-Sinica

基　　金：河北农业大学博士基金项目(2009-B-02)

摘　　要：为通过转基因技术改善草莓的贮运性能,以全明星草莓为试材,克隆了与草莓成熟有关的FaEtr2基因的2个片段,并构建了该基因的反义和正义植物表达载体。根据已克隆的草莓乙烯受体FaEtr2基因序列及表达载体pBI221上的酶切位点设计2对带限制性内切酶位点的特异性引物,以测序质粒为模板,PCR扩增到2个全明星草莓FaEtr2基因片段。两片段经双酶切消化后分别以正、反两个方向插入到植物表达载体pBI221的35 S启动子和NOS终止子之间,分别构建成All Star-FaEtr2基因的正、反义植物表达载体。Two fragments of All-star strawberry FaEtr2 gene were cloned using All-star strawberry as materials.Sense and antisense expression vector were constructed for improving storage and transport varieties of strawberry through transgenic technology.According to the restriction enzyme sites of expression vector and cloning sequence of strawberry FaEtr2 gene,two pairs of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid.PCR products and the plasmid pBI221 were digested by the corresponding restricted enzymes respectively,and linked directionally.Then the sense and antisense expression vector can be designed.The constructed expression vector was transformed into Agrobacterium LBA4404 for the following-up research.

关 键 词：草莓 乙烯受体 FaEtr2基因 植物表达载体 

分 类 号：Q78[生物学—分子生物学]