废水中除汞菌株洋葱假单胞菌抗汞质粒的分析  

Analysis of a Mercury-detoxification Pseudomonas cepacia Strain Plasmid

作　　者：高芬芳[1] 胡静平[2] 安艳[3] 

机构地区：[1]中国辐射防护研究院,山西太原030006 [2]苏州大学医学部基础医学与生物科学学院,江苏苏州215123 [3]苏州大学医学部放射医学与公共卫生学院,江苏苏州215123

出　　处：《环境科学与技术》2011年第1期38-40,共3页Environmental Science & Technology

基　　金：山西省自然科学基金项目(2008012004-1)

摘　　要：目前我国汞消费量日益增大且污染日益严重,汞污染已影响到我国居民的健康,含汞污染物一旦排放到环境中,造成的环境污染就会以食物链形式放大,危协人类的健康。利用微生物对含汞废水清除是一条适合我国国情的生物治理途径。通过研究发现环境中的某些微生物能利用自身的基因表达出一种汞还原酶及汞裂解酶,将环境污水中的汞离子还原成金属汞,降低汞的毒性。该实验通过对除汞微生物菌株的抗汞质粒基因进行研究,尤其是对它的目的基因片段利用PCR技术进行扩增研究,希望提高细菌清除环境中汞的能力。Environment is severely polluted with toxic heavy metal mercury.If mercuric industrial effluents were released in the biosphere,it can be amplified by food chain and have an effect on the people＇s health.Bioremediation is a kind of good method especially suitable to China.It was found that some of mercury-resistant bacteria detoxify chiefly by two induciable enzymes such as organomercurial lyase and mercuric reductase,acting respectively to cleave the carbon-mercury bond of certain organomercurial compounds and to reduce Hg to metallic mercury.The research on plasmid of the Pseudomonas cepacia strain especially on its plasmid gene by the method of polymerase chain reaction was done to facilitate the capacity of detoxification of mercury.

关 键 词：洋葱假单胞菌 质粒 汞 

分 类 号：X171.5[环境科学与工程—环境科学]