高效好氧反硝化细菌的筛选及脱氮特性的研究  

Screening of Highly Active Aerobic Denitrifiers and Its Denitrification Properties

作　　者：蔡昌凤[1] 梁磊[1] 

机构地区：[1]安徽工程科技学院生物化学工程系,安徽芜湖241000

出　　处：《环境科学与技术》2011年第1期41-44,共4页Environmental Science & Technology

基　　金：安徽省自然科学基金重点资助(08040102002)

摘　　要：由焦化废水生化处理站曝气池驯化后的活性污泥分离纯化得到三株好氧反硝化细菌,通过脱硝酸盐氮对比实验得到一株高效好氧反硝化细菌F1。分别在不同温度(20～40℃)、pH(5.5～8.0)、C/N比(白糖与KNO3质量比1.0～2.0)、焦化废水COD(500～1 000 mg/L)稀释液的条件下确定好氧反硝化优势菌脱氮的最佳条件,实验选出其最佳生物去除硝酸盐氮的温度为30～35℃、pH6.0～6.5,最佳C/N比为1.8;焦化废水COD的增加对好氧反硝化细菌F1脱硝酸盐氮能力有抑制作用。以白糖为碳源和电子供体,硝酸盐为唯一氮源,接种量为30%,起始硝酸盐氮194.1 mg/L、pH7.2±0.5、温度30℃条件下,连续曝气6 h后,F1对硝酸盐氮的去除率高达99.8%。Three strains of aerobic denitrifying bacteria were separated and purified from activated sludge in aerobic tank of a coking wastewater chemical and biological treatment station,a highly active oxygen denitrifying bacteria F1 was obtained by comparison experiment of de-NO3-N.The best biological removal of NO3--N was temperature 30～35 ℃,pH 6.0～6.5 and best C/N ratio 1.8.In the white sugar for the election donor and carbon source,nitrate as only nitrogen source,inoculums 30%,initial NO3--N 194.1 mg/L,pH（7.2±0.5）,temperature 30 ℃ and after 6 h aeration in a row,F1 on NO3--N removal rate was up to 99.8%.

关 键 词：焦化废水 好氧反硝化细菌 脱氮 

分 类 号：X131.2[环境科学与工程—环境科学]