星形细胞瘤体外化疗药物敏感程度的研究  

Study of chemosensitivity testing in vitro on astrocytoma

作　　者：滕晓华[1] 刘波[1] 周蓉[1] 曾年菊[1] 卢明[1] 

机构地区：[1]湖南师范大学第二附属医院解放军第一六三医院神经外科,长沙410003

出　　处：《中国医师进修杂志》2011年第2期7-10,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 了解星形细胞瘤对常用化疗药物的敏感程度,探讨化疗药物体外敏感试验对于胶质瘤化疗的指导意义,比较不同WHO分级星形细胞瘤对化疗药物敏感度的差异,为临床治疗提供依据.方法 收集手术切除的新鲜星形细胞瘤标本共142例,其中低级别星形细胞瘤（Ⅰ、Ⅱ级）55例,高级别星形细胞瘤（Ⅲ、Ⅳ级）87例,分别进行星形细胞瘤细胞分离培养,采用MTT法检测临床常用11种化疗药物对肿瘤细胞的生长抑制情况,判定药物敏感程度.结果 142例患者中有78例（54.93%）获临床推荐使用药物,其中低级别星形细胞瘤为41.82%（23/55）,而高级别星形细胞瘤为63.22%（55/87）.不同WHO分级星形细胞瘤敏感化疗药物基本相似,以替尼泊苷、卡铂、长春新碱更为敏感.结论 化疗药物体外敏感试验对于胶质瘤的化疗药物筛选具有一定的指导意义,恶性程度越高,作用越突出.不同WHO分级的星形细胞瘤在化疗药物的选择上差异不明显.Objective To observe the sensitivity ofastrocytoma to chemotherapeutic drugs, in order to explore the drug selection using chemosensitivity testing in vitro on astrocytoma. Comparing different WHO grade astrocytoma to chemotherapeutic drugs sensitivity differences,providing the basis data for clinical treatment. Methods The astrocytoma cells were seperated and cultured in vitro from 142 tumor tissues by glioma resection, including 55 cases of low-grade astrocytoma （WHO grade Ⅰ , Ⅱ） and 87 cases of high-grade astrocytoma （WHO grade Ⅲ, Ⅳ）. The proliferation inhibition and chemosensitivity of tumor cells to 11 drugs were investigated by using MTT method. Results There were 54.93% （78/142） patients recommended chemotherapeutic drug in 142 cases. The percentage was 41.82%（23/55） in the low-grade astrocytoma, and 63.22% （55/87） in the high-grade astrocytoma respectively. Teniposide,carboplatin,vincristine as the sensitive drugs were similar in different WHO grade astrocytoma. Conclusions The chemosensitivity testing could be used to filtrate sensitive drugs to clinical chemotherapy. The action is more prominent in high-grade astrocytoma than in low-grade astrocytoma. There is no difference in the choice of sensible drugs among different WHO grade astrocytoma.

关 键 词：星形细胞瘤 药物疗法 敏感性与特异性 

分 类 号：R739.4[医药卫生—肿瘤]