逆转录病毒载体介导的Anti-ras核酶对T24ras转化3T3细胞的抑制作用  

RETROVIRUS MEDIATED RIBOZYME TO INHIBIT TRANSFORMATION OF NIH3T3 CELLS BY THE ACTIVED RAS GENE

作　　者：赵立青[1] 史国利[1] 房新 陈雅文[1] 陈德风[1] 

机构地区：[1]南开大学分子生物学研究所

出　　处：《南开大学学报（自然科学版）》1999年第2期93-97,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市21世纪青年科学基金

摘　　要：突变ｒａｓ基因存在于多种人类肿瘤细胞中，本文将可特异性切割１２位点突变ｒａｓ（Ｔ２４ｒａｓ）之核酶Ｒ８通过逆转录病毒载体导入Ｔ２４ｒａｓ转化的ＮＩＨ３Ｔ３细胞，以期检测核酶在细胞内对其靶基因的作用．结果表明Ｒ８可特异性地切割突变ｒａｓｍＲＮＡ，导致转化细胞生物学特性如细胞形态。The involvement of mutations in the ras gene family in the genesis of a variety of human neoplasms has made it an attractive target for mutation compensation gene therapy strategies. We used a recombinant retrovirus vector to delive the ribozyme which cleave ras mRNA mutated at codon 12 in the transformed NIH3T3 cells. Expression of this ribozyme accomplished reversion of the neoplastic phenotype.

关 键 词：核酶 转化细胞 逆转录病毒 肿瘤细胞 基因治疗 

分 类 号：R730.5[医药卫生—肿瘤] R394[医药卫生—临床医学]