制何首乌多糖的脱蛋白研究  

Study on Deproteinization of Polysaccharide from Polygoni Multiflori Radix Preparata

作　　者：陈占科[1] 王淑美[2] 高珊[1] 黎瑞汝[2] 陈曦[2] 苏智斌[2] 梁生旺[2] 

机构地区：[1]河南中医学院,郑州450008 [2]广东药学院,广州510006

出　　处：《中国实验方剂学杂志》2011年第4期41-43,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:对制何首乌多糖进行脱蛋白处理,筛选出最佳的脱蛋白方法。方法:以多糖损失率和蛋白去除率为衡量指标,选用了Sevag法、三氯乙酸法和酶法对制何首乌多糖提取物进行脱蛋白处理。结果:酶法、三氯乙酸法和Sevag法的蛋白去除率分别为87.62%,73.12%,62.65%,多糖损失率分别为8.13%,29.36%,20.24%。结论:酶法除蛋白是去除制何首乌多糖提取液中蛋白质的较理想方法。Objective：To screen out the the optimal technology for deproteinization of polysaccharide from Radix Polygoni Multiflori Preparata.Method：The protein was removed by the enzyme,TCA and Sevage methods with the percentage of the removed protein（the PRP） and the decomposed polysaccharide（the PDP） as index.Result： The PRPs for the enzyme,TCA and Sevage methods were 87.62%,73.12% and 62.56%,the PDPs were 8.13%,29.36% and 20.24%.Conclusion：Enzymatic method of removing protein is an effective and alternative method.

关 键 词：制何首乌 多糖 酶 脱蛋白 

分 类 号：R283.6[医药卫生—中药学]