新城疫病毒感染过程中基质蛋白的动态分布  

Dynamic distribution of matrix protein in Class Ⅰ newcastle disease virus infection

作　　者：孙英杰[1] 陈鸿军[1] 詹媛[1] 于洋[1] 仇旭升[1] 宋翠萍[1] 于圣青[1] 丁铲[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海闵行200241

出　　处：《中国兽医杂志》2011年第1期15-18,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(30871883);2007年中央公益性研究院所基础业务专项经费(2007JB09)

摘　　要：本研究利用原核表达产物制备的多抗血清进行M蛋白在细胞内的定位分析,NDV 9a5b病毒按5个感染复数（m.o.i）感染量接种DF1单层细胞,当PI=6 h时,M蛋白主要分布于细胞浆内,并未到达细胞膜内侧;到PI=8 h时,粗面内质网周围的M蛋白逐渐增多;PI=12 h时,M蛋白已开始聚集于细胞膜的内侧,而当感染后16 h开始形成合胞体时,M蛋白在细胞膜内侧形成明显的斑点状聚集;当感染至24 h时,逐渐出现5~10个细胞膜融合的包涵体,M蛋白存在于包涵体融合膜的内层,且荧光强度已逐渐减弱。这为深入开展M蛋白与病毒增殖、宿主相互作用机制的研究工作奠定了基础。The matrix protein of Class Ⅰ newcastle disease virus was expressed in prokaryotic system.The antisera were collected from BALB/c mice immunized with the expressed antigen.The dynamic distribution of M protein was analyzed by the antisera on DF1 cells infected with 9a5b strain at a dose of 5 m.o.i.At PI=6 h,M protein distributed mainly in cytoplasm.At PI=8 h,M protein localized around the rough surfaced endoplasmic reticulum.At PI=12 h,the protein accumulated in the inner face of infected cell membranes.After 16h,the fusion of 5~10 cells induced the appearance of large inclusive bodies,and M protein localized in the inner face of the fused membranes,meanwhile the fluorescence intensity attenuated.The results provided the theory basis of how M protein plays a role in virus replication and assembly.It will be helpful to further study on the relationship between M protein and NDV proliferation.

关 键 词：新城疫病毒 ClassⅠ 基质蛋白 动态分布 

分 类 号：S852.659.5[农业科学—基础兽医学]