溶藻细菌YZ对铜绿微囊藻的溶藻特性研究  

Algicidal effect of an algae-lysing bacterium YZ on Microcystis aeruginosa

作　　者：牛丹丹[1] 郑青松[1] 刘兆普[1] 张娜[1] 汪辉[1] 姚瑶[1] 

机构地区：[1]南京农业大学资源与环境科学学院,江苏省海洋生物学重点实验室,江苏南京210095

出　　处：《中国环境科学》2011年第2期321-326,共6页China Environmental Science

基　　金：国家自然科学基金资助项目(30600086)

摘　　要：为了进一步研究本实验室前期从青岛一池塘分离出的溶藻细菌YZ的溶藻效应.考察了不同量的YZ无菌滤液对铜绿微囊藻生长量、叶绿素含量、丙二醛含量和超氧化物歧化酶活性、PSⅡ实际光能转化效率、最大相对电子传递效率、光能利用效率的影响.结果表明,在一定范围内,YZ溶藻效果与加入的无菌滤液的量成正比.当100mL中加入超过10mL的基础柠檬酸培养基时,铜绿微囊藻的生长即受到抑制.YZ无菌滤液使其丙二醛含量显著上升,微藻的SOD活力在处理6,72h时显著上升,溶藻细菌YZ滤液可以在72h内使藻细胞的PSⅡ实际光能转化效率、最大相对电子传递效率、光能利用效率等显著下降.YZ无菌滤液主要是通过降低保护酶活性、加大膜脂过氧化、显著抑制光合能力,起到对铜绿微囊藻的溶解作用。For the sake of further research on Algae-lytic effect of YZ which was isolated from a lake in Qingdao,The algae-lytic influences of different content of YZ bacteria-free filtrate on the growth of M.aeruginosa FACHB469,the content of chlorophyll and malondialdehyde（MDA）,superoxide dismutase（SOD） activity,the actual photochemical efficiency of PS Ⅱin the light（ΦPSⅡ=yield）,the maximal relative electron transport rate（Pm）,light use efficiency（α） in M.aeruginosa FACHB469were studied.In a certain range,the content of YZ bacteria-free filtrateYZ was in direct ratio with the effect of algae-lying.When more than 10mL bacteria-free filtrate was added into 100mL M.aeruginosa,it did not only inhibit the growth of FACHB469significantly,but also caused the MDA contents increased,the activity of SOD was enhanced in 6and 72hours.Besides,the yield,Pm and α were decreased obviously.Algae lysis of YZ was mainly attributed to the decreased activity of SOD,the enhancement in the liqid peroxidation of the cellular membrane and the photosynthetic inhibition of M.aeruginosa.

关 键 词：溶藻细菌YZ 铜绿微囊藻 溶藻 叶绿素荧光特性 

分 类 号：X172[环境科学与工程—环境科学]