一株产木聚糖酶嗜热菌的鉴定及酶学性质  

Identification of a thermophilic bacterium and preliminary characterization of the secreted xylanase

作　　者：陈学敏[1] 刘培培[1] 张波[1] 

机构地区：[1]北京联合大学应用文理学院,北京100191

出　　处：《微生物学通报》2011年第2期151-156,共6页Microbiology China

基　　金：国家973计划项目(No.2007CB707805)

摘　　要：从云南腾冲热泉水样中分离筛选得到一株产木聚糖酶的菌株。通过细菌形态观察、生理生化特性并结合16S rDNA序列分析,经鉴定,该菌为地芽孢杆菌(Geobacillus sp.),命名为Geobacillus sp.PZH1。对该菌株所产嗜热木聚糖酶及酶学特性进行了初步研究。SDS-PAGE和酶谱分析测得该酶分子量约为69 kD;酶的最适反应pH和最适反应温度分别为7.0和70°C,在pH 5.0-11.0和40°C-100°C范围内均有较高酶活;该酶在pH 5.0-12.0范围内和70°C以内具有较高的稳定性;40°C-100°C范围内,该木聚糖酶没有被检测到纤维素酶活性。The bacterium isolated from a water sample of Yunnan tengchong hot spring can secrete a kind of thermophilic xylanase.It was identified and named as Geobacillus sp.PZH1 by morphologic observation,physio-biochemical characteristics and 16S rDNA sequence alignment.Subsequently,its secreted xylanase and the xylanase's characteristics were researched preliminarily.SDS-PAGE electrophoresis and zymogram analysis suggested that the xylanase's molecular mass was 69 kD;the optimum pH and temperature of the partially purified enzyme were 7.0 and 70 °C respectively,and it performed noted activities from pH 5.0 to pH 11.0 and from 40 °C to 100 °C;it had high stability from pH 5.0 to pH 12.0 and under 70 °C;from 40 °C to 100 °C,no cellulase activity was detected for the partially purified xylanase.

关 键 词：嗜热菌 嗜热木聚糖酶 酶谱分析 纤维素酶活性 

分 类 号：TQ925[轻工技术与工程—发酵工程]