一株γ-聚谷氨酸合成菌的筛选与鉴定  

Screening and identification of a novel γ-polyglutamic acid producing strain

作　　者：姚俊[1] 陈宽婷[1] 魏钦俊[1] 阮文辉 曹新[1] 鲁雅洁[1] 

机构地区：[1]南京医科大学基础医学院生物技术系,江苏南京210029 [2]2山西省医药与生命科学研究院,山西太原030001

出　　处：《微生物学通报》2011年第2期164-168,共5页Microbiology China

基　　金：江苏省高校自然科学研究项目(No.09KJB530007)

摘　　要：从土壤中筛选分离获得一株γ-聚谷氨酸合成菌PGS-1,经鉴定为枯草芽孢杆菌(Bacillus subtilis),在富含谷氨酸和葡萄糖的培养基中可大量合成γ-聚谷氨酸,摇瓶发酵产量达26 g/L,不同于大多文献报道的微生物合成的γ-聚谷氨酸具有较高的分子量,该菌株合成的γ-聚谷氨酸分子量较低(3×105-4×105 kD),分子量分布较窄,可适用于低分子量要求的应用领域,如作为药物的控缓释载体,值得深入开发研究。A novel γ-polyglutamic acid(γ-PGA) producing-strain Bacillus subtilis PGS-1 was isolated from soil,which could produced a large mount of γ-PGA in the medium containing glucose and glutamate.The highest yield 26 g/L of γ-PGA was obtained in flask culture.Compared with other reported γ-PGA producing-strain,γ-PGA derived from Bacillus subtilis PGS-1 had a lower molecular weight(3×105-4×105 kD) and a narrower polydispersity(1.3-2.0),which could endow γ-PGA a promising application,such as in the drug delivery system.

关 键 词：Γ-聚谷氨酸 筛选 枯草芽孢杆菌 摇瓶发酵 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]