Alzheimer病患者海马神经元核仁组成的研究  

Investigation of the Nucleolar Organizer Regions in the Hippocampal Neurons of Alzheimer's Disease

作　　者：卢文甫[1] 汤洪川 范明[2] 王鲁宁[1] 贾建军 米瑞发[2] 

机构地区：[1]解放军总医院脑系科,100853 [2]军事医学科学院基础所

出　　处：《中国医学影像技术》1999年第7期512-514,共3页Chinese Journal of Medical Imaging Technology

摘　　要：目的　间接了解Ａｌｚｈｅｉｍｅｒ’ｓ 病（ＡＤ）海马神经元总体基因表达水平。方法　１０ 例ＡＤ 患者病理脑海马组织经Ｎｉｓｓｌ’ｓ 染色后，图像分析神经元核仁、核浆比值，核仁组成区银染色后图像分析神经元着色面积及积分吸光度，并与相应的正常青年组、老年组各１０ 例的海马标本进行比较对照。结果　老年组和ＡＤ组海马神经元核浆比值（ 分别为５４１ ±８１ 及５２８ ±９７） 下降与青年组（６０８ ±５９） 相比，差异有显著性（ Ｐ＜ ００５ 及００１） ；老年组与ＡＤ组相比，差异无显著性（ Ｐ＞ ００５） 。同时，老年组和ＡＤ组海马神经元核仁着色面积减少，积分吸光度减弱；老年组和ＡＤ组相比，ＡＤ组海马神经元核仁着色面积（２１１７ ±５４８ｕｍ） 及积分吸光度（１７３２４ ±４２８１） 相对增强，与老年组（ 分别为１８０６ ±６６１ｕｍ 与１４１４３ ±５４９９） 相比，差异有显著性（ 均为Ｐ＜ ００５） 。结论　Ｎｉｓｓｌ’ｓ 染色说明老年人和ＡＤ 患者海马神经元功能低下，核仁组成区银染色提示老年人和ＡＤ 患者神经元ｒＤＮＡ转录活性低下，但ＡＤ患者海马神经元转录活性相对增高，其细胞总体基因表达活性相对增强，此种细?Objective To determine indirectly the degree of total gene expression in the hippocampal neurons of Alzheimer's disease (AD).Methods Neuropathological examination was carried out in 10 cases of AD and control groups,including the Yang and elderly patients without any neurological disorder.The nucleoli nucleoplasm ration,stained area and intergral optical density of silver stained nucleolar orgnizer regions (NORS) in the hippocampal neurons studied by image analysis after Nissl's and silver stainings.Results The nucleoli nucleoplasm rations of hippocampal neurons were decreased in both of AD and elderly groups,in comparision with young group,with significant difference statistically ( P <0.05 and P <0.01 respectively).There was no significant difference between the AD and elderly group ( P >0.05) The stained area and intergral optical density in hippocampal neurons were also reduced in the AD and elderly groups as compared with young group.But the stained area and intergral optical density of hippocampal neurons were increased more significantly in the AD group than that in the elderly group ( P <0.05).Conclusions The results of nucleoli nucleoplasm rations indicated that the hippocampal neuron functions in the AD and elder were declined because of degenerative process of neurons.The results of silver stained NoRs suggested that more active translating activity of rDNA in the AD hippocampal neurons might represent the cell defence function although the translating activity was diminished in the AD and elderly groups. [

关 键 词：核仁组成区 基因 早老性痴呆 海马 

分 类 号：R749.160.2[医药卫生—神经病学与精神病学]