重组葡激酶突变体(r-SAKΔN)的纯化  

Purificaion of Recombinant Staphylokinase Mutant(rSAKN)

作　　者：叶立文[1] 任军[1] 宋后燕[1] 

机构地区：[1]上海医科大学分子遗传研究室

出　　处：《药物生物技术》1999年第2期65-69,共5页Pharmaceutical Biotechnology

摘　　要：重组葡激酶突变体基因在大肠杆菌中高效表达，产物以包涵体的形式存在于胞浆中。通过发酵，扩增工程菌，机械破菌，离心分离粗制包涵体，后者经纯化后用盐酸胍溶解，ｒ－ＳＡＫΔＮ分子复性后用离子交换层析和分子筛脱盐，纯度达９７％，比活性５×１０４ＨＵ／ｍｇ，Ｍｒ１４０００，等电点为ｐＨ６．８。Recombinant staphylokinase mutant (rSAKN) was efficiently expressed in E coli in the form of inclusion body By the methods of fermentation, inclusion body extraction, refolding of rSAKN molecular, Qsepharose FF ionexchange chromatography and gel filtration of Sephadex G25 The purity of rSAKN was reached 97% and with specific activity 5104 HU/mg The Mr and isoelectro point were 14000 and pH68 respectively

关 键 词：葡激酶 突变体 包涵体 纯化 

分 类 号：Q78[生物学—分子生物学] TQ464.7[化学工程—制药化工]