胰岛素对兔形觉剥夺性近视眼光学参数及组织病理学的影响  

Effects of insulin on ocular optical parameters and histopathology of form deprived myopic rabbit

作　　者：高前应[1] 高如尧[1] 王培杰[2] 郭延奎[3] 卢佩勇[1] 蒙艳春[1] 朱涛[1] 李力[1] 

机构地区：[1]山东省泰安解放军第88医院眼科,271000 [2]山东省泰安解放军第88医院病理科,271000 [3]山东农业大学实验中心

出　　处：《眼视光学杂志》1999年第2期77-79,共3页Chinese Journal of Optometry & Ophthalmology

摘　　要：目的：探索实验性近视的发病机制，并观察胰岛素对近视形成的影响。方法：采用形觉剥夺方法，在幼兔眼建立近视动物模型。取7日龄幼兔52只,分A、B、C三组。A组20只，为单纯缝合；B组16只，为缝合眼睑＋玻璃体内注射胰岛素;C组16只，为缝合眼睑＋玻璃体内注射生理盐水，第60天用A超测量双眼的前房深度、晶体厚度、玻璃体腔长度和眼轴长度，并计算玻璃体腔长度／眼轴长度，同时进行病理学观察。结果：在三组中形觉剥夺能导致实验眼相对性近视，两眼的玻璃体胶长度、眼轴长度和玻璃体胶长度／眼轴长度间的差异均有高度显著的统计学意义（P＜0．001），而前房深度和晶体厚度无明显统计学差异．病理学也有明显改变，巩膜胶原纤维变细，视网膜色素上皮层改变是最突出的变化。结论：形觉剥夺能导致眼球的轴性延长，使眼球的形态学和组织学发生改变，其中玻璃体腔延长。巩膜胶原纤维的变细、延长和视网膜色素上皮层改变起着非常重要的作用，而胰岛素不能阻止这些改变。To study the possible role of insulin in the development of myopia. Methds: 52 seven - day rab-bits, which were divided into three groups A, B and C and were monoctilary deprived(MD) of pattem visi0n by sutamhon ofrigllt eyebds. In Group A, no clIug was used. In Group B and C, Anmch received a series of intravitreal injection of insulin(n= l6) or sallne (n = l6) in hght eyes, whereas left eyes were employed as control. Anteri0r chamber depth(ACD), lenhculartheckness(LT),vitreous ch^r length(VCL) and drial length(AL) were detendned f0r all eyes with A ultrsound 0n thetoth day, VCUAL was calculated and hist0pathologic changes were also ohserved. Redts: F0rm deprivah0n can induce rel-ative myopia in all groups, and VCL'AL and VCI/AL, but not ACD and LT, were significangly different betWeen experimentaland control eyes. Moreover, obvious histopathologic changes were also found. Conclusion: Form deprivahon can induce axialenlongment. Longer vitreous chamber thinner scleral fibre and changes of the retinal pigment epithelitun, which could not haprevented by insulin.

关 键 词：胰岛素 形觉剥夺 近视 病理学 

分 类 号：R779.110.2[医药卫生—眼科]