肝细胞糖原拮抗肝脏缺血再灌注损伤及其相关机理的研究  

EFFECT OF INTRACELLULAR GLYCOGEN ON LIVER ISCHEMIAREPERFUSION AND ITS MECHANISM

作　　者：汤礼军[1] 田伏洲[1] 王雨[1] 李晓军[1] 尹致良[1] 

机构地区：[1]成都军区总医院全军普外中心实验室

出　　处：《中国普外基础与临床杂志》1999年第4期196-198,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：为了探讨增加细胞内糖原含量能否改善肝脏缺血再灌注损伤程度，本实验对３组糖原含量显著不同的兔肝脏缺血再灌注过程中的组织形态学、肝脏酶学、组织ＡＴＰ含量、细胞膜Ｃａ２＋ＡＴＰ酶活性及胞浆内游离Ｃａ２＋浓度进行了观察。结果：糖原含量高的肝脏，其细胞能量代谢旺盛，细胞膜Ｃａ２＋ＡＴＰ酶活性强，胞浆内游离Ｃａ２＋浓度相对稳定，组织结构及功能损伤轻。本实验结果提示：缺血前增加肝脏糖原含量可显著地拮抗肝脏缺血再灌注损伤。To investigate the effect of intracellular glycogen on liver ischemiareperfusion and its mechanism, histomorphological and enzymological changes, histic ATP contents, the activity of Ca2+ATPase on cytoplasmic membrane and intracellular free Ca2+concentration were observed during the ischemiareperfusion of three groups of rabbit livers with different glycogen content. We found that the more vigorous energy metabolism, the higher activity of Ca2+ATPase, the lower concentration of intracellular free Ca2+ and the slighter injury of histic structure and function appeared in the liver with the more abundant glycogen. The results suggest that intracellular abundant glycogen may reduce liver ischemiareperfusion injury.

关 键 词：肝脏 糖原 肝缺血 再灌注损伤 

分 类 号：R575.02[医药卫生—消化系统]