逆转录聚合酶链反应技术检测人肝癌nm23-H1基因mRNA表达  

mRNA EXPRESSION OF nm23H1 GENE IN HUMAN LIVER TUMOR ASSAYED BY REVERSE TRANSCRIPTIONPOLYMERASE CHAIN REACTION

作　　者：徐骁[1,2] 郑树森[1,2] 陈智 梁廷波[1,2] 张珉[1,2] 黄东胜 

机构地区：[1]浙江医科大学附属第一医院肝胆外科 [2]浙江医科大学传染病研究所

出　　处：《中国普外基础与临床杂志》1999年第4期204-206,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：为探讨人肝癌中肿瘤转移抑制基因ｎｍ２３Ｈ１的ｍＲＮＡ表达状况，设计特异性引物，应用逆转录聚合酶链反应（ＲＴＰＣＲ）技术检测分析了２０例人肝癌组织及相应癌旁肝组织中ｎｍ２３Ｈ１基因的ｍＲＮＡ表达。结果：特异性引物能成功扩增ｎｍ２３Ｈ１基因的几乎整个编码序列；２０例肝癌及癌旁肝组织的ＲＴＰＣＲ结果均为阳性，ｎｍ２３Ｈ１基因ｍＲＮＡ未发生表达缺失或较大变异现象。由此表明：本实验建立的ＲＴＰＣＲ方法为进一步定量测定肝癌组织中ｎｍ２３Ｈ１ｍＲＮＡ表达水平奠定了基础。To investigate the mRNA expression of nm23H1 gene in human liver tumor. In tumor and corresponding nontumoral liver specimens from 20 patients, nm23H1 mRNA were examined by reverse transcriptionpolymerase chain reaction (RTPCR) method with specific primers. Results: The primers designed in this study could amplified nearly entire coding sequence of nm23H1 gene. All the samples showed positive expression of nm23H1 mRNA, indicating there was no expression loss or obvious alteration. Conclusions: The achievement of RTPCR method lays foundation for quantitative gaugement of nm23H1 mRNA in liver tumor.

关 键 词：肝肿瘤 聚合酶链反应 NM23-H1基因 mRNA 

分 类 号：R735.7[医药卫生—肿瘤]