Effects of Sublethal Doses of Chlorfluazuron on Embryogenesis in Spodoptera fitura  

作　　者：F. Perveen 

机构地区：[1]Department of Zoology, Hazara University, Garden Campus, Mansehra 21300, Northern Pakistan

出　　处：《Journal of Agricultural Science and Technology》2011年第2期127-138,共12页农业科学与技术（美国大卫英文）

摘　　要：This paper describes effects of sublethal-doses of chlorfluazuron on morphology of embryogenesis when LD10 （1.00 ng/larva） or LD30 （3.75 ng/larva） was applied topically to newly-ecdysed fifth-instars of common cutworm, Spodoptera litura F.. Experiments were conducted on eggs of F1 generation laid by subsequent adults under laboratory conditions. Fecundity was significantly reduced by LD10 and more significantly reduced by LD30 compared with untreated females. Number of unfertilized-unhatched-eggs was not significantly affected by the treatments, Fertilized eggs revealed changes in external coloration of eggs. Embryonic development of S. litura was divided into eight embryonic stages. During stage 5 （12-16 h after oviposition）, in LD10-treated-egg, crescent-shaped-embryo was affectedly differentiated. During the same stage, it was seriously affected in LD30-treated-egg. During stage 7 （32-64 h after oviposition）, in LD10-treated-egg, organogenesis was established, but body structures were affectedly developed. In LD30-treated-egg, body structures were more affectedly developed during the same stage. It is concluded that embryonic developmental stages were affected by LD10 and more seriously affected by LD30. The effects of residualchlorfluazuron on embryogenesis are thought to be one of the factors responsible for the reduction in fertility and hatchability caused by sublethal doses of chlorfluazuron in S. litura.

关 键 词：CHLORFLUAZURON ECDYSTEROID nucleic acids PROTEIN Spodoptera litura. 

分 类 号：S[农业科学]