RP-HPLC法测定辣椒中辣椒素、二氢辣椒素和降二氢辣椒素含量  

RP-HPLC simultaneous determination of three capsaicinoids constituents in fruits of Capsicum annuum

作　　者：张晶[1] 孙长波[1] 石磊岭[1] 金莎[1] 韩玉珠[2] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林农业大学园艺学院,长春130118

出　　处：《药物分析杂志》2011年第2期244-246,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：吉林省教育厅资助项目(No.2008-63)

摘　　要：目的:建立反相高效液相色谱法同时测定辣椒中辣椒素、二氢辣椒素和降二氢辣椒素含量。方法:采用Nucleosil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 mL.min-1,检测波长280 nm,柱温45℃,外标法定量。结果:3个辣椒碱的线性关系良好,线性范围均为1.0～160.0μg.mL-1,相关系数在0.9991～0.9995之间;精密度、稳定性试验的RSD均低于3%;辣椒素、二氢辣椒素和降二氢辣椒素平均回收率(n=6)分别为96.4%,98.1%,99.3%,RSD分别为2.6%,2.1%,1.2%;检出限分别为0.2,0.1,0.1μg.mL-1。结论:该方法简便快捷,应用本方法测定了10个品种辣椒中3个辣椒碱的含量,结果稳定。Objective：To develop a reserved-phase HPLC method for the determination of capsaicin,dihydrocapsaicin and nordihydrocapsaicin in fruits of Capsicum annuum.Methods：Nucleosil C18（4.6 mm×250 mm,5 μm） was used at 45 ℃ with the mobile phase of acetonitrile-water（gradient elution）.The flow rate was set at 1.0 mL·min-1,and the detection wavelength was 280 nm.Results：The linear response ranged from 1.0 to 160.0 μg·mL-1 with coefficient correlation of 0.9991-0.9995;RSDs of precision and stability test were lower than 3%;The average recoveries were 96.4% with RSD of 2.6% for capsaicin,98.1% with RSD of 2.1% for dihydrocapsaicin and 99.3% with RSD of 1.2% for nordihydrocapsaicin.Detection limits of capsaicin,dihydrocapsaicin and nordihydrocapsaicin are 0.2,0.1,0.1 μg·mL-1 respectively.Conclusion：The method is quick,simple and repeatable for determination of three capsicine constituents in fruits of 10 Capsicum annuum varieties.

关 键 词：高效液相色谱法 辣椒 辣椒素 二氢辣椒素 降二氢辣椒素 

分 类 号：R917[医药卫生—药物分析学]