甲拌磷残留检测间接竞争ELISA试剂盒的研制  

Development of Indirect ELISA Kit for Detecting Phorate Residue

作　　者：魏松红[1] 逄若霖[1] 王翀[1] 纪明山[1] 谷祖敏[1] 祁之秋[1] 王英姿[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《食品科学》2011年第4期284-287,共4页Food Science

基　　金：沈阳农业大学中、青年硕士生导师资助项目

摘　　要：研制用于检测甲拌磷残留量的间接竞争ELISA试剂盒。甲拌磷包被抗原的最佳工作质量浓度为4mg/L,抗体的最佳稀释倍数为8000,甲拌磷多克隆抗体交叉反应率小于20%,甲拌磷抑制率回归曲线为y=18.846x+6.9949,在1～5000μg/L范围内呈线性关系,检测限为4.90μg/L,IC50为191.37μg/L。甲拌磷试剂盒的添加回收率均高于75%,供试样品检测结果的批内和批间变异系数均低于10%。试剂盒在4℃或-20℃下有效期为270d。An indirect enzyme-linked immunosorbent assay（ELISA）kit was developed to monitor phorate residue. The optimal concentration of coating antigen was 4 mg/L and the optimal dilution ratio of antibody was 8000. The cross-reaction ratio was less than 20%. The regression equation of inhibition rate for phorate was y = 18.846x＋6.9949 with a linear detection range of 1-5000μg/L. Results indicated that the detection limit was 4.90μg/L and IC50 was 191.37μg/L. The recovery rates were higher than 75%. The intra-assay and inter-assay coefficients were less than 10%. The developed kit can be stored at 4℃ or-20 ℃ for 270 days.

关 键 词：甲拌磷 残留 酶联免疫吸附测定法 试剂盒 

分 类 号：S482.4[农业科学—农药学]