大鼠单侧输尿管完全梗阻后肾脏HGF的表达及意义  

Expression and significance of HGF in rat kidney with complete unilateral ureteral obstruction

作　　者：陈凯飞[1] 任来成[1] 陈明明[1] 田智谋[1] 

机构地区：[1]山西医科大学第二医院泌尿外科,太原030001

出　　处：《中国医疗前沿》2011年第1期27-29,共3页China Healthcare Innovation

摘　　要：目的观察大鼠单侧输尿管完全梗阻(complete unilateral ureteral obstruction,CUUO)后不同时期肝细胞生长因子(hepatocyte growth factor,HGF)表达的变化,探讨HGF在肾脏间质纤维化发病机制中的意义。方法建立CUUO模型,采用半定量逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)检测和免疫组化法检测(1周、2周、4周)梗阻侧和代偿侧肾脏及假手术对照组HGFmRNA和HGF蛋白的表达。结果 CUUO模型梗阻侧和代偿侧肾脏HGFmRNA及HGF蛋白的表达在输尿管结扎后1周、2周、4周不同时间点均显著高于对照组肾脏(P<0.05)。随着梗阻时间的延长,HGFmRNA(P<0.05)和HGF蛋白(P<0.05)逐渐增加。而且对于同一个实验对象代偿侧要远远高于梗阻侧HGFmRNA(P<0.05)和HGF蛋白(P<0.05)。结论在整体条件下随着肾脏间质纤维化程度的加重,HGF在有功能的残余肾脏组织中表达逐渐增强,提示HGF可能在肾脏纤维化的发病机制中起重要的作用。Objective To investigate the role of HGF activation pathway in the development of renal tubule interstitial fibrosis in vivo.Methods Rats were sacrificed after1 weeks,2 weeks,and 4 weeks of complete unilateral ureteral obstruction（CUUO）,and sham-operated rats were sacrificed as the control.HGFmRNA and protein and were analyzed with semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and Immunohistochemistry.Results HGFmRNA and protein levels of the CUUO group at the 1 weeks,2 weeks,4 weeks in the kidneys of rats with obstructed kidneys and compensatory kidneys,were significantly increased（P0.05）,compared to sham-operated rats.HGFmRNA expression were peaked 7 days after CUUO（P0.05）.HGF protein expression was peaked 1 week after CUUO.The levels of HGF protein expression were peaked 4 weeks after CUUO（P0.05）.Conclusion Expressions of HGF are increasted in renal tissue after CUUO.HGF signaling pathway may be involved in CUUO rats and plays a concordant role in the pathogenesis of renal interstitial fibrosis.

关 键 词：输尿管梗阻 肾间质 肝细胞生长因子 

分 类 号：R365[医药卫生—病理学] R692[医药卫生—基础医学]