hPTTG1基因沉默下调bFGF表达并抑制A2780细胞侵袭的研究  

The inhibition of hPTTG1 siRNA on the ability of invasion by downregultaion of bFGF in ovarian cancer

作　　者：刘洁[1] 张军[2] 刘峥嵘[3] 卢瑶[1] 赵恂[1] 

机构地区：[1]中国医科大学实验技术中心,辽宁沈阳110001 [2]中国医科大学盛京医院放射科,辽宁沈阳110004 [3]辽宁省人民医院普外科,辽宁沈阳110016

出　　处：《现代肿瘤医学》2011年第2期221-225,共5页Journal of Modern Oncology

基　　金：辽宁省科学技术基金博士启动基金(编号:20081042)

摘　　要：目的:探讨RNAi技术沉默卵巢癌细胞A2780细胞内hPTTG1表达对其侵袭性的影响及调控机制。方法:利用阳离子转染试剂将hPTTG1 siRNA转染A2780细胞,荧光实时定量PCR检测hPTTG1及bFGF表达水平;MTT基质黏附实验检测细胞黏附能力;细胞侵袭重建基底膜实验测定细胞体外侵袭能力;划痕实验检测细胞迁移能力;Western blot检测bFGF蛋白表达水平。结果:hPTTG1siRNA转染组hPTTG1mRNA表达下降,抑制率为(76.8±4.1)%;转染hPTTG1siRNA后细胞黏附、侵袭和迁移能力降低;hPTTG1干扰后bFGFmRNA及蛋白表达下降。结论:hPTTG1siRNA能够抑制A2780细胞侵袭,可能是通过下调bFGF表达实现的。Objective：To investigate the role of hPTTGlsiRNA on the invasiveness of ovarian cancer A2780 and the molecular mechanism . Methods： hPTFG1siRNA was transfected into A2780 by lipofectamine. The expression level of hPTTG1, bFGF mRNA were examined by fluorescent real - time quantitative PCR. The ability of cell adhesion,invasion and migration were analyzed by MTT adhension assay, transwell chamber experiment and scratch assay. The expression level of bFGF protein was detected by Western blot. Results ： The expression of hPTFG1 mRNA was inhibited by hPTTGIsiRNA and the inhibitory efficiency was （76.8 ±4.1 ）%. The ability of cell adhesion, invasion and migration were decreased after hPTFGI siRNA transfection. The mRNA and protein activity of bFGF were down - regulated. Conclusion ： The invasive ability of A2780 was inhibited by hPTFG1 siRNA by downregulating the expression of bFGF.

关 键 词：HPTTG1 卵巢癌 侵袭 BFGF 

分 类 号：R730[医药卫生—肿瘤] R737.31[医药卫生—临床医学]