5-羟基癸酸盐对慢性低氧大鼠肺动脉Kv1.5通道表达的影响  

Effects of 5-hydroxydecanoate on Kv1.5 expression in pulmonary arteries of chronic hypoxic rats

作　　者：李秋[1] 张丽萍[1] 舒鹰[1] 李云雷[1] 陈成水[1] 

机构地区：[1]温州医学院附属第一医院呼吸内科,浙江温州325000

出　　处：《中国病理生理杂志》2011年第1期22-26,共5页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金资助项目(No.Z2080988)

摘　　要：目的:以大鼠为研究对象,研究线粒体ATP敏感性钾通道(mitoKATP)的抑制剂5-羟基癸酸盐(5-HD)对慢性低氧肺动脉高压大鼠的影响及其潜在机制。方法:24只SD雄性大鼠随机分成对照组、低氧组、低氧+5-羟基癸酸盐干预组,每组8只。将低氧组和5-HD干预组大鼠放入常压低氧舱内[O2(10.0%±0.3%)]以建立低氧肺动脉高压模型。4周后测定平均肺动脉压(mPAP)及右心室与左心室及室间隔的重量比[RV/(LV+S)],并采用RT-PCR及Western blotting技术,分析各组肺动脉Kv1.5 mRNA及蛋白表达。结果:(1)慢性低氧组大鼠的mPAP及RV/(LV+S)显著高于正常对照组(P<0.05),5-HD干预组mPAP及RV/(LV+S)显著低于低氧组,均P<0.05。(2)低氧组Kv1.5通道mRNA及蛋白表达显著低于正常组,5-HD组Kv1.5通道表达显著高于低氧组,均P<0.05。结论:mitoKATP通道的抑制剂5-HD通过降低mPAP及RV/(LV+S),在慢性低氧肺动脉高压中起保护作用。mitoKATP通道的抑制及Kv1.5通道表达的上调可能与该保护作用有关。AIM：To investigate the effect of mitochondrial ATP-sensitive potassium channel（mitoKATP） inhibitor 5-hydroxydecanoate（5-HD） on chronic hypoxic pulmonary artery hypertension（CHPAH） in rats.METHODS：Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups：control group,hypoxia group and hypoxia＋5-HD group.To establish an animal model of CHPAH,the rats in hypoxia group and hypoxia＋5-HD group were placed into a hypoxic and normobaric chamber [O2（10.0±0.3）%] for 4 weeks（8 h a day,6 days a week）.The mean pulmonary arterial pressure（mPAP） and the ratio of right ventricle weight to left ventricle with septum weight [RV/（LV＋S）] were measured.Western blotting and reverse transcription-polymerase chain reaction（RT-PCR） were performed to analyze the protein and mRNA levels of Kv1.5 in the pulmonary artery,respectively.RESULTS：The mPAP and RV/（LV＋S） were much higher in hypoxia group than those in control group,while those in hypoxia＋5-HD group were decreased as compared to hypoxia group.The protein and mRNA levels of Kv1.5 in hypoxic group were significantly lower than those in control group,and this decrease was partly reversed by 5-HD.CONCLUSION：5-HD may exert a protective effect on CHPAH by decreasing mPAP and RV/（LV＋S）.Inhibiting mitoKATP and stimulating Kv1.5 expression by 5-HD may contribute to the mechanism of this effect.

关 键 词：5-羟基癸酸盐 KV1.5通道 线粒体ATP敏感性钾通道 大鼠 

分 类 号：R363[医药卫生—病理学]