抑制肿瘤血管新生的复合肽VBP3局部刺激与全身过敏性实验  

Security assessment of complex peptide vaccine VBP3 for anti-tumor angiogenesis:analysis of local irritation and systemic anaphylaxis

作　　者：康艳丽[1] 王宏[1] 向军俭[1] 王盼盼[1] 吕卫东[1] 邓宁[1] 

机构地区：[1]暨南大学抗体工程研究中心,广东广州510632

出　　处：《中国病理生理杂志》2011年第1期113-118,共6页Chinese Journal of Pathophysiology

基　　金：广州市科技攻关资助项目(No.2006Z2-E4051);广东省生物工程药物重点实验开发基金资助项目(No.51206001)

摘　　要：目的:评价抑制肿瘤血管新生的复合肽VBP3的安全性。方法:同体左右侧自身对比法对3只家兔进行皮下注射的局部刺激实验。28只豚鼠随机分为4组:无菌生理盐水(PS)阴性对照组6只,牛血清白蛋白(BSA)阳性对照组6只,复合肽VBP3低剂量组6只,复合肽VBP3高剂量组6只,进行全身过敏实验;剩余4只豚鼠,牛血清白蛋白阳性对照组和复合肽VBP3高剂量组各2只,不经致敏直接心脏激发。结果:抑制肿瘤血管新生的复合肽VBP3对家兔皮下注射的局部刺激反应轻微;豚鼠实验仅复合肽VBP3高剂量组1只豚鼠出现弱阳性过敏反应,且很快缓解,其余豚鼠未见过敏症状。结论:抑制肿瘤血管新生的复合肽VBP3在本实验条件下安全。AIM：To evaluate the security of an anti-tumor angiogenesis complex peptide vaccine VBP3.METHODS：Three rabbits were tested for local irritation by local subcutaneous injection of the vaccine with a self comparison method on the left/right sides of the same body.The test of systemic anaphylaxis was performed in 28 guinea pigs,in which the animals were divided into 4 groups：normal saline（NS） group,bovine serum albumin（BSA） group,low-dose vaccine group and high-dose vaccine group.The other 4 guinea pigs,2 received BSA and 2 received vaccine at a high dose,were activated directly without sensitization.RESULTS：No irritable and allergic reaction was observed in the test of all rabbits with local subcutaneous injection and most of the guinea pigs with systemic anaphylactic test.Only one guinea pig with slightly allergic response was found and spontaneously recovered soon.CONCLUSION：The complex peptide vaccine VBP3 for anti-tumor angiogenesis is safe under the experimental conditions.

关 键 词：血管内皮细胞生长因子 成纤维细胞生长因子2 复合肽疫苗VBP3 

分 类 号：R363[医药卫生—病理学]