乳腺癌骨转移瘤中干细胞样肿瘤细胞的分离及其MDR1、ABCG2的表达研究  

Isolation of cancer stem-like cells from bone metastasis of breast cancer and expression of MDR1,ABCG2

作　　者：李迅[1] 杨顺娥[1] 白靖平[2] 江仁斌[2] 何祖胜[2] 焦谊[3] 龚磊[2] 

机构地区：[1]新疆医科大学附属肿瘤医院内三科 [2]新疆医科大学附属肿瘤医院骨与软组织肿瘤科 [3]新疆医科大学基础医学院分子生物学教研室,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第1期43-47,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区高校科研计划青年教师培育基金(XJEDU2008S36)

摘　　要：目的对乳腺癌骨转移瘤干细胞样肿瘤细胞(类SP细胞)进行分选,检测MDR1、ABCG2在乳腺癌骨转移瘤细胞分选亚群中的蛋白表达差异。方法应用Rho123结合流式细胞分选术(FCA)对乳腺癌骨转移原代细胞进行Rho123low和Rho123high亚群分选,采用western-blot方法检测MDR1、ABCG2蛋白在2个亚群中的表达状况。结果在乳腺癌骨转移瘤原代细胞中存在Rho123low和Rho123high2个亚群,2个亚群细胞中MDR1、AB-CG2蛋白表达差异有统计学意义(P<0.05)。结论利用Rho123结合FCA可在乳腺癌骨转移瘤原代细胞中分选出具有干细胞特性的Rho123low亚群(类SP细胞)。Objective To detect the difference expression level of MDR1,ABCG2 from two subpopulations of bone metastatic from breast cancer using the methods of sorting stem-like cells（like side population cells） from bone metastaisis cells of breast cancer.Methods Flow cytometry analysis（FCA） combination with Rho123 were used to depart primary cancer cells of bone metastatic from breast.The expression of MDR1,ABCG2 in the two subgroups were detected by Western-blot.Results After using Rho123 staining,there were two Rho123low and Rho123high in bone metastasis from breast cancer.The expression of MDR1,ABCG2 exsited significantly differences between Rho123low and Rho123high subgroup cells（P0.05）.Conclusion FCA combination with Rho123 can enrich and isolate the subgroup Rho123low which have characteristics of stem cells in bone metastasis tumor from breast cancer.

关 键 词：骨转移瘤 罗丹明123 流式细胞仪 肿瘤干细胞 MDR1 ABCG2 

分 类 号：R737.9[医药卫生—肿瘤]