不同营养水平对羔羊肌肉组织学性状的影响  

Effects of Different Nutritional Levels on Muscle Histological Characteristics of Lambs

作　　者：张崇志[1] 高爱武[2] 侯先志[1] 考桂兰[3] 刘小刚[1] 张园[1] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]内蒙古农业大学兽医学院,呼和浩特010018

出　　处：《动物营养学报》2011年第2期336-342,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(30860194)

摘　　要：本试验旨在研究羔羊在营养限制期和补偿期背最长肌、半腱肌和股二头肌的肌纤维直径、面积和密度的变化规律。选用蒙古羔羊(羯羊)80只,随机分为4个组,即:对照组(C组)、中等受限1组(R1组)、中等受限2组(R2组)、严重受限组(R3组),进行营养限制期60 d和补偿期90 d的羔羊生长试验。限制期4个组饲粮能氮水平分别为代谢能(ME):10.88、10.88、9.41、8.62 MJ/kg,粗蛋白质(CP):15.00%、10.00%、10.00%、5.70%;补偿期各组饲喂同一能氮水平(ME 9.75 MJ/kg,CP 12.00%)的饲粮。结果表明:限制期结束时,R1和R2组羔羊肌纤维直径和面积高于C组和R3组,但R1和R2组羔羊的肌纤维密度低于C组,极显著低于R3组(P<0.01)。补偿期结束时,4个组羔羊肌纤维直径、面积、密度差异均不显著(P>0.05)。研究表明:饲粮中能量和蛋白质水平影响羔羊肌纤维的直径、面积和密度。在限制期,当饲粮ME为8.62 MJ/kg、CP为5.7%时,羔羊的肌纤维发育受到严重影响,而在补偿期肌纤维发育快,明显表现出补偿生长效应。This experiment was conducted to investigate the effects of different nutritional levels on muscle histological characteristics of lambs.Eighty castrated Mongolian lambs were randomly divided into four equal groups.Lambs in the control group（group C） were fed a diet（ME 10.88 MJ/kg,CP 15.00%） ad libitum;lambs in the moderate restriction groups（groups R1 and R2） were fed restriction diets（ME 10.88 MJ/kg,CP 10.00% and ME 9.41 MJ/kg,CP 10.00%,respectively）;lambs in the severe restriction group（group R3） were fed a restriction diet（ME 8.62 MJ/kg,CP 5.70%）.The lambs were fed restriction diets for 60 days followed by compensation diet（ME 9.75 MJ/kg,CP 12.00%） for 90 days.The changes in the diameter,cross section area and density of muscle fibers in longissimus dorsi,semitendinosus and biceps femoris of the lambs in each group were measured in the restriction and compensation period.The results showed that the muscle fiber diameter and cross section area of groups R1 and R2 were higher than those of groups C and R3.However,the muscle fiber density of groups R1 and R2 was lower than that of group C,and significantly lower than that of group R3（P0.01） with feed restriction.There were no significant differences in the diameter,cross section area and density of muscle fiber among the four groups by the end of the compensation period（P0.05）.The results indicate that the energy and protein levels of the diets significantly affect the diameter,cross section area and density of muscle fibers in lambs.The development of muscle fiber in lambs is severely affected by the nutritional levels of the diet（ME 8.62 MJ/kg,CP 5.70%） during the restriction period.However,the muscle fiber develops rapidly,and presents compensatory growth effects during the compensation period.

关 键 词：营养限制 营养补偿 肌纤维直径 肌纤维面积 肌纤维密度 

分 类 号：S826[农业科学—畜牧学]