三氧化二砷对神经母细胞瘤细胞增殖的影响  

Preliminary studies of arsenic trioxide on the proliferationof neuroblastoma cells

作　　者：伍钢[1,2] 周云峰[1,2] 应大明 陈同辛[1,2] 王耀平 林梓[1,2] 

机构地区：[1]湖北医科大学附属二院放化疗科 [2]上海第二医科大学附属新华医院

出　　处：《癌症》1999年第3期263-265,共3页Chinese Journal of Cancer

基　　金：国家自然科学基金

摘　　要：目的：观察三氧化二砷体外对神经母细胞瘤细胞增殖的影响及其作用机制的探讨。方法：①ＭＴＴ法观察三氧化二砷对神经母细胞瘤细胞生长的影响；②化学染色观察实验和对照组细胞形态学改变；③ＰＩ染色后流式细胞仪检测实验和对照组细胞凋亡峰；④凝胶电泳观察凋亡细胞ＤＮＡ的“梯型”条带。结果：①三氧化二砷明显抑制神经母细胞瘤细胞株ＳＪＮＳＨ细胞增殖，抑制作用呈剂量和作用时间依赖性；②通过细胞形态学、流式细胞仪对ＤＮＡ的测定及ＤＮＡ凝胶电泳均证实，三氧化二砷可诱导ＳＪＮＳＨ细胞发生凋亡。在一定浓渡范围内，凋亡率与三氧化二砷的浓度和作用时间呈依赖关系。结论：三氧化二砷可明显抑制神经母细胞瘤细胞增殖。Objective: To investigate the effect of arsenic trioxide(As2O3) on the proliferation of neuroblastoma (NB) cells and its mechanisms of action. THZMethods: 1The effect of As2O3 on the proliferation of NB cells were measured with MTT method. 2Morphological changes of the experimental and control groups were observed under the optical microscope in MayGrundwalGiemsa stain.3Ratio of apoptotic cell was determined by the flow cytometry assay in propidium iodide staining.4DNA fragmentation was observed on the gel electrophoresis. Results: 1As2O3 could significantly inhibit the proliferation of NB cell line SJNSH and the inhibitory effect was doseandtimedependent.2All results of morphology,the flow cytometry assay and the gel electrophoresis indicated that As2O3 induce apoptosis of SJNSH cells.Besides, that As2O3induced apoptosis of SJNSH cells was doseandtimedependent. Conclusion: As2O3 significantly inhibited the proliferation of SJNSH cells through inducing apoptosis in these cells.

关 键 词：三氧化二砷 神经母细胞瘤 细胞凋亡 细胞增殖 

分 类 号：R730.264[医药卫生—肿瘤]