P27及VEGF在膀胱移行细胞癌中的表达及临床意义  

P27,VEGF in bladder rransitional cell carcinoma and its clinical significance

作　　者：梁豪[1] 李志军[1] 张建国[1] 程远合[1] 孙国贤[1] 肖飞[1] 王晓辉[1] 王晓彬[1] 

机构地区：[1]河南科技大学第一附属医院泌尿外科,河南洛阳471003

出　　处：《中国肿瘤外科杂志》2011年第1期14-16,共3页Chinese Journal of Surgical Oncology

摘　　要：目的研究细胞周期抑制蛋白P27和血管内皮生长因子(vascular endothelial growth factor,VEGF)在膀胱移行细胞癌(bladder transitional cell carcinoma,BTCC)组织中的表达及临床意义。方法运用免疫组化SP法检测60例BTCC和10例正常膀胱组织中P27及VEGF的表达。结果 (1)P27蛋白主要在细胞的细胞核中表达,其在BTCC组中的阳性表达率为20.0%,在正常组织中的阳性表达率为80.0%,差异有统计学意义(P<0.05);P27在膀胱移行细胞癌中的表达随肿瘤病理分级与临床分期的升高而降低。(2)VEGF主要在肿瘤细胞膜和胞质中表达,其在BTCC组中的阳性表达率为81.7%,在正常组织中的阳性表达率为10.0%,差异有统计学意义(P<0.05);VEGF表达随肿瘤病理分级与临床分期的升高而增高。结论膀胱移行细胞癌中P27的明显下调以及VEGF的明显上调可能与膀胱移行细胞癌的发生、发展及转移有关,检测二者的表达有助于综合判断膀胱移行细胞癌的生物学行为。Objective To investigate the expression of P27 and VEGF in bladder transitional cell carcino- ma. Methods The expression of P27 and VEGF was detected by immunohistochemistry in 60 cases of BTCC and 10 cases of normal bladder tissue. Results （ 1 ） P27 protein was mainly expressed in the cell nucleus, positive rate in the BTCC group was 20% , normal tissues was 80% , the difference was significant （ P 〈 0.05 ）. With the tumor grade and stage increased, P27 expression in bladder transitional cell carcinoma was significantly reduced; （2） VEGF was mainly expressed in the tumor cell membrane and cytoplasm, positive rate in the BTCC group was 81.7% , normal tissues was 10% , the difference was significant （P 〈0.05）. With the tumor grade and stage increased, P27 expression in bladder transitional cell carcinoma was significantly increased. Conclusions The upregulation of P27 and downregulation of VEGF in BTCC may be associated with the devel- opment and metastasis of carcinoma. Detection the expression of P27 and VEGF can determine the biological behavior of BTCC.

关 键 词：细胞周期抑制蛋白P27 血管内皮生长因子 膀胱移行细胞癌 蛋白表达 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]