肝细胞癌组织中丙肝病毒抗原及HBsAg的免疫组化检测  

Immunohistochemical Detection of Hepatitis C Virus Antigen from Hepatocellular Carcinoma

作　　者：周凤[1] 赵连三[1] 刘聪[1] 雷学忠[1] 周思亮[1] 王锦蓉[1] 

机构地区：[1]华西医科大学附属第一医院病毒性肝炎研究室

出　　处：《华西医科大学学报》1999年第2期126-127,132,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金

摘　　要：为了解丙肝病毒（ＨＣＶ）感染与我国肝细胞癌（ＨＣＣ）之间的关系，以及ＨＣＶ抗原成分（ＨＣＡｇ的）在ＨＣＣ组织中的分布状况，应用鼠抗ＨＣＶ单克隆抗体，采用免疫组化技术检测ＨＣＣ组织中ＨＣＡｇ的表达，同时检测了乙肝病毒表面抗原（ＨＢｓＡｇ）。结果：４０例ＨＣＣ标本组织中ＨＣＡｇ、ＨＢｓＡｇ检出率分别为１７．５％（７／４０）和７０％（２８／４０），其中单一ＨＣＡｇ阳性者仅１例，ＨＣＡｇ和ＨＢｓＡｇ同时存在的检出率为１５％（６／４０）。镜下见ＨＣＡｇ呈细颗粒状分布于细胞胞浆，ＨＣＡｇ阳性细胞以局灶型分布为主。提示我国部分肝癌患者的癌组织中确实存在ＨＣＶ，ＨＣＶ感染可能是除乙肝病毒以外，导致我国ＨＣＣ发生的另一相关因素。To investigate the infection of HCV and the expression of HCV antigen (HCAg) in HCC, immunohistochemical protocol was performed on tumorous liver tissues from 40 patients with HCC to detect HCAg and hepatitis B virus surface antigen (HBsAg) simultaneously.The results showed that the positive rates for HCAg and HBsAg were 17.5% (7/40) and 70% (28/40) respectively. HBsAg and HCAg were simultaneously detected in six of 40 cases and only one case had HCAg positive alone in the liver tumorous tissue. The positive signals were localized in diffuse cytoplasm of hepatocytes and the positively stained cells were mainly distributed in local pattern. The results suggested that HCV infection did exist in some cases of HCC, so HCV might be a viral risk factor in addition to HBV in the genesis of HCC in China.

关 键 词：肝肿瘤 肝细胞癌 丙型肝炎病毒 抗原 HBSAG 

分 类 号：R735.702[医药卫生—肿瘤] R373.21[医药卫生—临床医学]