Skp2与P27kipl在基底细胞癌及其临床病理特征中的表达  

Expression of P27kipi and Skp2 in basal cell carcinoma and its clinical pathological characters

作　　者：代大毛 朱辉[1] 

机构地区：[1]深圳市北京大学深圳医院整形外科汕头大学医学院 ,518036

出　　处：《国际肿瘤学杂志》2011年第2期158-160,共3页Journal of International Oncology

摘　　要：目的 检测S期激酶相关蛋白2（Skp2）与激酶抑制蛋白1（P27kip1）在基底细胞癌（BCC）中的表达,进而探明两者在BCC发生发展中的作用及其相关性,以及与临床病理特征的关系,并初步探讨Skp2与P27kip1在BCC 临床分型中的作用,尤其在硬化型中的作用.方法用免疫组织染色SP两步法检测50例BCC及30例正常皮肤组织中Skp2与P27kip1的表达情况,并结合患者的年龄、性别及临床分型等进行综合分析.结果 Skp2定位于胞核或胞质,而P27kip1只定位于胞核.Skp2在BCC中呈高表达,而P27kip1呈低表达,与正常皮肤组织对照组比较,差异有统计学意义（P＜0.05）.Skp2和P27kip1的异常表达与BCC的临床分型有关（P＜0.05）,而与患者性别、年龄等因素均无关（P＞0.05）.在BCC中,Skp2的阳性表达与P27kip1的阳性表达呈负相关（r=-0.624;P＜0.05）;但在BCC的硬化型中,两者无相关性（P＞0.05）.结论 Skp2与P27kip1在BCC中的阳性表达呈负相关,提示在BCC的发生及发展中两者可能起协同作用,并可能为将来的治疗提供靶点.Skp2在硬化型BCC组的表达高于结节型及浅表型BCC组,而P27kip1则相反,提示Skp2与P27kip1可能参与皮肤BCC的分型,且可能成为BCC分型的预测因子,从而为不同类型BCC手术切缘的设计提供生化指标依据.Objective To investigate the expression of Skp2 and P27kip1 and their relationship with the clinicopathologic characters in basal cell carcinoma（BCC）. To explore their expression between with the types of BCC of skin, especially in morpheaform BCC group. Methods Immunohistochemical SP technique was used to examine the expression of Skp2 and P27kip1 in 50 cases of BCC and 30 cases of normal tissues of skin.The expression level was analyzed combined with clinicopathological features of age, sex and types of tumor.Results Skp2 positive products located in cytoplasm or nucleus. P27kip1 positive products located in nucleus.The high expression of Skp2 and the low expression of P27kip1 were observed in BCC, there was statistical significance between the tumor group and the control group （P 〈 0. 05 ） . The expression of Skp2 and P27kip1 were correlated with clinical types of BCC （ P 〈 0. 05 ）, while which were not correlated with age and sex （ P 〉0.05 ）. In BCC tissue, Skp2 expression was negatively correlated with P27kip1 （ - 0.624; P 〈 0.05）, there was no correlated with each other in morpheaform BCC group （ P 〉 0. 05 ）. Conclusion The expression of Skp2 and P27kip1 in BCC are significant differences to normal skin tissues, and they are correlated with each other.They may play an important role in carcinogenesis and the development of BCC, and also make targets to treat it. Compared to nodular and superficial BCC group, the expression of Skp2 are higher in morpheaform BCC group, and the expression of P27kip1 is lower. They may play an important role in the types of the BCC and useful factors to predict the types of the tumor, also determine surgical margin for BCC cases.

关 键 词：肿瘤 基底细胞 S期激酶相关蛋白质类 周期素依赖激酶抑制剂P27 

分 类 号：R739.5[医药卫生—肿瘤]